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Protocol to request

Preparation of Mcm2-7^4SNAP549, Mcm2-7^2SORT549 Mcm2-7^4SNAP5496ΔN, Mcm2-7^{4SNAP549-6AD/E+ASP/Q}, and Mcm2-7^{2SORT549-4AD/E+ASP/Q}

Research article

DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7
Lorraine De Jesús-Kim et al.

DOI: 10.7554/eLife.65471

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