Protocol to request
            
                Assays for carbamoyl phosphate synthetase activity and allosteric regulation
                
                
            
        
        
            
                Research article
            
            
                Mutations that improve efficiency of a weak-link enzyme are rare compared to adaptive mutations elsewhere in the genome
                
            
            
            
                Andrew B Morgenthaler et al.
            
            
                DOI: 10.7554/eLife.53535