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Protocol to request

Expression and purification of EGFP–helix, EGFP–helix (5E), GRAMD1a_StART, GRAMD1b_StART, GRAMD1b_StART T469D and 5P mutants of GRAMD1a_StART and GRAMD1b_StART

Research article

Movement of accessible plasma membrane cholesterol by the GRAMD1 lipid transfer protein complex
Tomoki Naito et al.

DOI: 10.7554/eLife.51401

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