Request a detailed protocol

As part of our efforts to make science more reproducible, we're working with Bio-protocol to link the protocols they host with research published at eLife. To request a protocol for an eLife paper that's not yet available on Bio-protocol, fill in the form below and they will send a request to the author.

If the author chooses to make it available, the Bio-protocol team will let you know via email and a link will be added to the eLife article. Learn more about requesting a protocol.

Please do not request that authors share details that are already available in their research article.

Protocol to request

Expression and purification of EGFP–helix, EGFP–helix (5E), GRAMD1a_StART, GRAMD1b_StART, GRAMD1b_StART T469D and 5P mutants of GRAMD1a_StART and GRAMD1b_StART

Research article

Movement of accessible plasma membrane cholesterol by the GRAMD1 lipid transfer protein complex
Tomoki Naito et al.

DOI: 10.7554/eLife.51401

Your name

Please enter your name

Your email address

Please enter your email address

Your message to the authors Tips for writing messages

Please include a message to authors

Post your question anonymously to a public Q&A (optional).

This will add your question to a Q&A on the Bio-protocol journal's sister site, Bio-protocol Exchange, so that others can benefit from the discussion of this protocol.

By sending your request you are consenting to eLife passing your details to Bio-protocol and for Bio-protocol to contact you via email. Your details and message will also be sent to the corresponding author. Please read the privacy policy for more details.