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Protocol to request

Antibodies, protein extracts, immunoprecipitation, western blotting, cell fractionation, chromatin immunoprecipitation (ChIP) and kinase assay

Research article

The CDK Pef1 and protein phosphatase 4 oppose each other for regulating cohesin binding to fission yeast chromosomes
Adrien Birot et al.

DOI: 10.7554/eLife.50556

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