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Protocol to request

Labelling of single Trp, single Cys-containing moPrP with non-fluorescent acceptor thionitro benzoate (TNB), dinitrophenyl (DNP) or fluorescent 5-((((2-Iodoacetyl)amino)ethyl)amino)Naphthalene-1-Sulfonic Acid) (DANS)

Research article

Monitoring site-specific conformational changes in real-time reveals a misfolding mechanism of the prion protein
Ishita Sengupta et al.

DOI: 10.7554/eLife.44698

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