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Published: Jul 20, 2011 DOI: 10.21769/BioProtoc.95 Views: 65269
Abstract
Transient expression in tobacco plant (Nicotiana benthamiana) is used to determine the subcellular location of a protein of interest when tagged with a reporter such as green fluorescent protein (GFP), or to mass produce proteins without making transgenic plants. The root tumor bacteria, Agrobacteria, are used as medium to introduce the target gene expression cassette into benthamiana mesophyll cells.
Keywords: Transient expressionMaterials and Reagents
Equipment
Procedure
Category
Plant Science > Plant transformation > Agrobacterium
Biochemistry > Protein > Expression
Molecular Biology > Protein > Expression
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