Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Peer-reviewed
Extract Genomic DNA from Arabidopsis Leaves (Can be Used for Other Tissues as Well)
Published: Jul 5, 2011 DOI: 10.21769/BioProtoc.90 Views: 30610
PDF 
Ask a question
How to
cite
Favorite
Cited by
Abstract
This is a simple protocol for isolating genomic DNA from fresh plant tissues. DNA from this experiment can be used for all kinds of genetics studies, including genotyping and mapping. This protocol uses Edward’s extraction buffer to isolate DNA.
Materials and Reagents
- Ethanol (EtOH)
- Isopropanol
- NaCl
- EDTA
- SDS
- Tris (pH 7.5)
- Edward buffer (see Recipes)
Equipment
- Ceramic mortar and pestles
- Centrifuges (Eppendorf)
- Vortex (VWR International)
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Molecular Biology > DNA > DNA extraction
Plant Science > Plant molecular biology > DNA > DNA extraction
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question2 Q&A
