- Home
- Protocols
-
Extract Genomic DNA from Arabidopsis Leaves (Can be Used for Other Tissues as Well)
Published: Jul 5, 2011 DOI: 10.21769/BioProtoc.90 Views: 30581
Download PDF
Ask a question
Abstract
This is a simple protocol for isolating genomic DNA from fresh plant tissues. DNA from this experiment can be used for all kinds of genetics studies, including genotyping and mapping. This protocol uses Edward’s extraction buffer to isolate DNA.
Materials and Reagents
- Ethanol (EtOH)
- Isopropanol
- NaCl
- EDTA
- SDS
- Tris (pH 7.5)
- Edward buffer (see Recipes)
Equipment
- Ceramic mortar and pestles
- Centrifuges (Eppendorf)
- Vortex (VWR International)
Procedure
Category
Molecular Biology > DNA > DNA extraction
Plant Science > Plant molecular biology > DNA
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question
