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Extract Genomic DNA from Arabidopsis Leaves (Can be Used for Other Tissues as Well)
Published: Jul 5, 2011 DOI: 10.21769/BioProtoc.90 Views: 30589
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Abstract
This is a simple protocol for isolating genomic DNA from fresh plant tissues. DNA from this experiment can be used for all kinds of genetics studies, including genotyping and mapping. This protocol uses Edward’s extraction buffer to isolate DNA.
Materials and Reagents
- Ethanol (EtOH)
- Isopropanol
- NaCl
- EDTA
- SDS
- Tris (pH 7.5)
- Edward buffer (see Recipes)
Equipment
- Ceramic mortar and pestles
- Centrifuges (Eppendorf)
- Vortex (VWR International)
Procedure
Category
Molecular Biology > DNA > DNA extraction
Plant Science > Plant molecular biology > DNA
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