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Thioglycollate Induced Peritonitis
Published: Jun 20, 2011 DOI: 10.21769/BioProtoc.84 Views: 33451
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Abstract
Intraperitoneal (i.p.) injection of thioglycollate elicits a robust influx of neutrophils into peritoneal cavity. The trafficking of the cells is believed to be mediated by chemokines CXCL1, CXCL2, and CXCL8 (Call et al., 2001; Cacalano et al., 1994). Thus this model can be used to test the ability of neutrophils to migrate towards these chemokines in bioengineered mouse strains (e.g. knockout or transgenic mice) or the ability of certain molecules to inhibit the chemoattractive activities of these chemokines (e.g. small molecules or inhibitory antibodies). This protocol has been used by the author successfully to test the functions of a viral multi-chemokine inhibitor.
Keywords: Mouse model
Peritonitis
Inflammation
Thioglycollate
Background
Materials and Reagents
- Antibodies
- Other materials
- Mice
- PBS
- 4% sterile thioglycollate (Sigma-Aldrich, catalog number: 70157 ) in ddH2O
Note: Thioglycollate solution needs to be wrapped with aluminum foil to avoid light and be placed at room temperature to age for several weeks until it turns to brown in color. The aging process is critical to the ability of thioglycollate to induce peritonitis.
- Mice
Equipment
- 6G1/2 needle
- 18G1/2 needle connected with a 10 ml syringe
- BD LSR II flow cytometer
Procedure
Category
Immunology > Animal model > Mouse
Immunology > Immune cell function > Cytokine
Cell Biology > Cell movement > Cell migration
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