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Standard PCR Protocol
Published: Apr 5, 2011 DOI: 10.21769/BioProtoc.53 Views: 40010
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Abstract
This protocol describes basic steps of a PCR experiment using home-made Taq DNA polymerase. Some steps may vary with different DNA polymerase.
Materials and Reagents
- Tris-HCl (Sigma-Aldrich)
- KCl (EM SCIENCE)
- MgCl2 (EM SCIENCE)
- Gelatin (Sigma-Aldrich)
- Taq DNA polymerase (home-made)
- dNTPs (New England Biolabs, catalog number: N0447L )
- Template DNA (genomic, plasmid, cosmid, bacterial/yeast colony, etc.)
- Primers
Equipment
- Thermal cycler (MJ Research)
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Molecular Biology > DNA > PCR
Molecular Biology > DNA > DNA cloning
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