- Home
- Protocols
-
siRNA Transfection of Mouse Bone Marrow-derived Macrophages
Published: Feb 5, 2011 DOI: 10.21769/BioProtoc.28 Views: 24116
Download PDF
Ask a question
Abstract
Short interfering RNAs (siRNA) are a type of double-stranded RNA molecule, typically 20-25 bp long, that are involved in the phenomenon of RNA interference. siRNA transfection is employed in this protocol to knockdown target gene expression in BMM’phi’ cells. Two days after transfection with cells at 60-80% confluence, the knockdown efficiency can reach 90%.
Keywords: Macrophage
Transfection
SiRNA
Materials and Reagents
- RPMI 1640 medium (RPMI) (Life Technologies, InvitrogenTM, catalog number: 11875-093 )
- Fetal bovine serum (Atlanta Biologicals, catalog number: S10350 )
- Stock penicillin/streptomycin (P/S) (Life Technologies, InvitrogenTM, catalog number: 15140-122 )
- Lipofectamine RNAiMAX (iMAX) (Life Technologies, InvitrogenTM, catalog number: 13778150 )
Equipment
- Cell counter
- 6-well plate
- 24-well plate
Procedure
Category
Immunology > Immune cell function > Macrophage
Molecular Biology > RNA > Transfection
Molecular Biology > RNA > RNA interference
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question
