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Protocol for knockdown of HuR with siRNA

FL FengZhi Liu

Published: May 20, 2012 DOI: 10.21769/BioProtoc.217 Views: 12072

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Abstract

Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.

Materials and Reagents

  1. HuR-siRNA (Life Technologies, Ambion®, catalog number: 4390824 )
  2. Control siRNA (Life Technologies, Ambion®, catalog number: Am4611 )
  3. OPITMEM (Life Technologies, InvitrogenTM, catalog number: 3198 )
  4. Oligofectamine (Life Technologies, InvitrogenTM, catalog number: 12252-011 )
  5. Culture medium
  6. Dulbecco's modified eagle medium (DMEM)
  7. Water (RNase free)

Equipment

  1. 6-well plate
  2. Flask T-75
  3. Gloves

Procedure

Copyright: © 2012 The Authors; exclusive licensee Bio-protocol LLC.

Category

Cancer Biology > General technique > Biochemical assays > RNA

Molecular Biology > RNA > RNA interference

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This post has helped me think things trohguh
edit 0 Answer 6 Views Nov 16, 2012