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Cell Culture Mycoplasma Detection by PCR
Published: Apr 20, 2012 DOI: 10.21769/BioProtoc.207 Views: 19968
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Abstract
DNA was extracted from the supernatant of each sample. After PCR-amplification of mycoplasma DNA, detection was performed by gel electrophresis. The PCR primers were designed to cover the consensus sequences that can detect all types of mycoplasma species.
Background
Materials and Reagents
- Ampli Taq Gold (Life Technologies, InvitrogenTM, catalog number: 4338856 )
- Sodium acetate
- Ethanol
- Phenol
- MgCl2
- PCR buffer
- Sodium acetate
- dNTPs
- Agarose gel
- TE-saturated phenol
- Ethidium bromide
- DDW
- Stock solution A (see Recipes)
- Master mixture A (see Recipes)
Equipment
- 1.5 ml Eppendorf tube
- Centrifuges
- Micropipette
Procedure
Copyright: © 2012 The Authors; exclusive licensee Bio-protocol LLC.
Category
Molecular Biology > DNA > PCR
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