- Home
- Protocols
-
Purification of Adenovirus by Cesium Chloride Density Gradients
Published: Apr 5, 2012 DOI: 10.21769/BioProtoc.201 Views: 34259
PDF 
Ask a question
Abstract
Adenovirus are efficient gene delivery systems. The standard method for purification of adenoviral vectors is based on using a cesium chloride (CsCl) density gradient combined with ultracentrifugation. This method is suitable for small-scale purification and is less expensive than column chromatography or commercial purification kits.
Materials and Reagents
- HEK293 cell
- Glycerol
- Liquid nitrogen
- 70% ethanol
- Distilled water
- SDS
- CaCl2
- MgCl2
- CsCl2 (Thermo Fisher Scientific, catalog number: BP1595-500 )
- Tris-HCl (pH 8.0)
- TE
- EDTA
- SDS
- Sucrose
- 5% deoxycholate (see Recipes)
- Dialysis buffer (see Recipes)
- Balance buffer (see Recipes)
- Saturated CsCl2 (see Recipes)
Equipment
- Centrifuges
- Ultracentrifuge
- Laminar flow hood
- Syringe
- Water bath
- Slide-A-Lyzer 10 K dialysis cassette (Thermo Fisher Scientific, catalog number: 66203 )
- Spectrometer
- Ti50.2 tube
Procedure
Category
Microbiology > Microbial cell biology > Cell isolation and culture
Microbiology > Microbe-host interactions > Virus
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question
