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Cryopreservation of Human Serum

YR Yael Rosenberg-Hasson
Holden Maecker Holden Maecker

Published: Nov 5, 2015 DOI: 10.21769/BioProtoc.1648 Views: 8751

Original research article

The authors used this protocol in:

Human Immune Monitoring Center (HIMC)

14-Oct 2013

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Abstract

This protocol describes how to collect and aliquot human serum and store at -80 °C for future cytokine/chemokine/protein profile analysis.
Please use personal protective equipment (PPE) as required when handling samples with potential Bloodborne Pathogens.

Keywords: Serum search Cryopreservation search Collection & Processing search Storage search Clot activator search

Background

Materials and Reagents

  1. Red top tube (BD, catalog numbers: 366431 , 366441 and 366430 )
  2. Cryovials
  3. Patient whole blood sample in red top tube
  4. Clot activator (optional)

Equipment

  1. Freezing box
  2. -80 °C freezer
  3. Biosafety cabinet
  4. Centrifuge

Procedure

Copyright: © 2015 The Authors; exclusive licensee Bio-protocol LLC.

Category

Immunology > Immune cell isolation > Maintenance and differentiation

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