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Activation of ER-regulated Fusion Proteins In Vivo
Published: Dec 20, 2011 DOI: 10.21769/BioProtoc.164 Views: 12569
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Abstract
Regulatable protein fusions can be made by attaching the hormone binding domain of the estrogen receptor to the N or C-terminus of a protein of interest. Activation of such ER-fusion proteins in vivo can be achieved by daily administration of a synthetic ligand, 4-hydroxytamoxifen (4OHT), via intraperitoneal (i.p.) injection.
Background
Materials and Reagents
- Corn oil (Sigma-Aldrich, catalog number: C8267 )
- 200-proof ethanol (Sigma-Aldrich, catalog number: E7023 )
- 4OHT (Sigma-Aldrich, catalog number: H7904 )
Equipment
- Sonic dismembrator (Thermo Fisher Scientific, model: 100 )
- Centrifuges
- Hybridization oven
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Molecular Biology > Protein > Expression
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