Escherichia coli Outer Membrane Vesicle Immunization Protocol and Induction of Bacterial Sepsis
利用大肠杆菌外膜囊泡诱发细菌脓毒症的模型
发布: 2013年12月05日第3卷第23期 DOI: 10.21769/BioProtoc.994 浏览次数: 12892
评审: Anonymous reviewer(s)
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Apr 2013
Abstract
Outer membrane vesicles (OMVs) are spherical bilayered phospholipids of 20-200 nm in size produced from all Gram-negative bacteria and Gram-positive bacteria investigated to date. OMVs, which resemble the outer membrane and periplasm in composition, are proinflammatory and immunogenic facsimiles, and therefore could activate both innate and adaptive immunity. Here, we describe the OMVs immunization protocol and bacteria challenge protocol to induce bacterial sepsis in mice.
Keywords: Outer membrane vesicles (外膜囊泡)
Sepsis (脓毒症)
Animal model (动物模型)
Bacterial extracellular vesicles (细菌胞外囊泡)
Immunization (免疫)
Materials and Reagents
- Five-week-old male C57BL/6 or BALB/c mice or a variety of transgenic/knockout mice, body weight 18-20 g
- Phosphate buffered saline (PBS) (Gibco®, catalog number: 70013-032 )
- Escherichia coli (E. coli) derived OMVs
- E. coli (Isolated from the peritoneal lavage fluid of cecal ligation and puncture-operated mice)
- Luria-Bertani broth (LB) medium (Merck KGaA, catalog number: 1.10285.0500 )
Equipment
- 1.5 ml microtube
- Ultra-fine-II insulin syringe 1 ml 31 G (0.25 mm x 8 mm) (BD, catalog number: 328820 )
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Kim, O. Y., Hong, B. S., Park, K., Yoon, Y. J., Choi, S. J., Lee, W. H., Roh, T., Kim, Y. and Gho, Y. S. (2013). Escherichia coli Outer Membrane Vesicle Immunization Protocol and Induction of Bacterial Sepsis. Bio-protocol 3(23): e994. DOI: 10.21769/BioProtoc.994.
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分类
免疫学 > 动物模型 > 小鼠
微生物学 > 微生物-宿主相互作用 > 细菌
微生物学 > 微生物-宿主相互作用 > 体内实验模型 > 哺乳动物
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