Analysis of Moraxella catarrhalis Outer Membrane Protein Profiles
卡他莫拉菌外膜蛋白图谱分析
发布: 2013年11月05日第3卷第21期 DOI: 10.21769/BioProtoc.957 浏览次数: 9083
评审: Fanglian He
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参见作者原研究论文
The authors used this protocol in:
Jan 2013
Abstract
Phenotypes observed for certain Moraxella catarrhalis wild-type strains or mutants may be caused by a variety of factors including alteration of outer membrane protein composition. Examination of the outer membrane protein profiles may be a valuable tool to identify changes in outer membrane compositions of these strains. Here we describe a method to isolate and analyse M. catarrhalis fractions highly enriched for membrane proteins.
Keywords: Moraxella catarrhalis (卡他莫拉菌)
Outer membrane vesicles (外膜囊泡)
Pathogenesis (发病机制)
Virulence (毒力)
Materials and Reagents
- Brain heart infusion (BHI) (Becton Dickinson and Company, catalog number: 237500 ) broth and BHI agar plates
- Antibiotics
e.g. spectinomycin or kanamycin (Merck KgaA, Calbiochem, catalog numbers: 567570-10 and 420311-5 )
- PBS
- Bovine skin gelatin (Sigma-Aldrich, catalog number: G9382-100G )
- ReadyPrep Protein Extraction Kit (membrane I) (Bio-Rad, catalog number: 1632088 )
- Glass beads, acid-washed (150-212 μm) (Sigma-Aldrich, catalog number: G1145 )
- 2D-Quant Kit (General Electric Company, catalog number: 80-6483-56 )
- Mini-protean TGX precast gels, 4-15% (Bio-Rad, catalog number: 456-1083 )
- Colloidal Coomassie staining (Pink et al., 2012)
Equipment
- CO2 incubator
- Benchtop Incubator Shaker
- Centrifuge
- TissueLyser LT (QIAGEN, model: 85600 )
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Vries, S. P. D. and Bootsma, H. J. (2013). Analysis of Moraxella catarrhalis Outer Membrane Protein Profiles. Bio-protocol 3(21): e957. DOI: 10.21769/BioProtoc.957.
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分类
微生物学 > 微生物蛋白质组学 > 膜蛋白
系统生物学 > 蛋白质组学 > 外膜蛋白质
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