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Adhesion of Moraxella catarrhalis to Respiratory Tract Epithelial Cells

卡他莫拉菌在呼吸道上皮细胞的粘附试验

Stefan P.W. de Vries Stefan P.W. de Vries
Hester J. Bootsma Hester J. Bootsma

发布: 2013年11月05日第3卷第21期 DOI: 10.21769/BioProtoc.956 浏览次数: 9779

评审: Fanglian He

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Cover of Molecular Microbiology, featuring study using the protocol.

Jan 2013

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Abstract

Moraxella catarrhalis is a human-restricted pathogen that is responsible for respiratory tract infections such as childhood otitis media (OM) and exacerbation of chronic obstructive pulmonary disease (COPD) in adults. Successful colonization and infection by M. catarrhalis depends on its ability to attach to the respiratory tract mucosal epithelium. This protocol describes a method to measure adherence of M. catarrhalis to epithelial cell lines in vitro.

Keywords: Moraxella catarrhalis (卡他莫拉菌) search Epithelial cells (上皮细胞) search Adhesion (粘附) search Respiratory tract (呼吸道) search Pathogenesis (发病机制) search

Materials and Reagents

  1. Human pharyngeal epithelial cell line Detroit 562 (ATCC, catalog number: CCL-138 )
  2. Type II alveolar epithelial cell line A549 (ATCC, catalog number: CCL-185 )
  3. DMEM + GlutaMAXTM-I (Life Technologies, Invitrogen™, catalog number: 31966-047 )
  4. Fetal calf serum (FCS) (Greiner Bio-One GmbH, catalog number: 758093S5403 )
  5. Trypsin-EDTA (0.25%-1 mM) (Life Technologies, catalog number: 25300-054 )
  6. Brain heart infusion (BHI) (Becton Dickinson and Company, catalog number: 237500 ) broth and BHI agar plates
  7. Antibiotics: spectinomycin or kanamycin (Merck KgaA, Calbiochem, catalog numbers: 567570-10 and 420311-5 )
  8. Bovine skin gelatin (Sigma-Aldrich, catalog number: G9382-100G )
  9. Glycerol (Merck KgaA, catalog number: 1.04092.1000)
  10. PBS without Ca2+ and Mg2+ (Westburg BV, catalog number: BE17-516F/12 )
  11. Saponin (Sigma-Aldrich, catalog number: 47036-50G-F )

Equipment

  1. 24-well tissue culture plate (Falcon®, catalog number: 353407 )
  2. 75 cm2 culture flask
  3. Centrifuge
  4. CO2 incubator
  5. Benchtop Incubator Shaker

Procedure

English
中文翻译

文章信息

版权信息

© 2013 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Vries, S. P. D. and Bootsma, H. J. (2013). Adhesion of Moraxella catarrhalis to Respiratory Tract Epithelial Cells. Bio-protocol 3(21): e956. DOI: 10.21769/BioProtoc.956.

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分类

微生物学 > 微生物-宿主相互作用 > 体外实验模型 > 细胞系

细胞生物学 > 细胞结构 > 细胞粘附

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