Improve Research Reproducibility A Bio-protocol resource
  • search
  • 提交稿件
  • 订阅
  • CN change language
Peer-reviewed

Adhesion of Moraxella catarrhalis to Respiratory Tract Epithelial Cells

卡他莫拉菌在呼吸道上皮细胞的粘附试验

Stefan P.W. de Vries Stefan P.W. de Vries
Hester J. Bootsma Hester J. Bootsma

发布: 2013年11月05日第3卷第21期 DOI: 10.21769/BioProtoc.956 浏览次数: 9356

评审: Fanglian He

download pdf 下载 PDF 下载 PDF
ask Q&A
引用
收藏
Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of Molecular Microbiology, featuring study using the protocol.

Jan 2013

Presubmission Inquiry

实验方案合集

专注于特定技术或应用的、详细的、经过同行评审的实验方案合集

Cancer Research
Immunology
Developmental Biology
Microbiology
Neuroscience
Cell Imaging - A Special Collection for Cell Bio 2023
查看更多 more

相关实验方案

γ-H2AX作为标志物采用细胞内蛋白质印迹法（ICW）定量HeLa细胞中Colibactin相关基因毒性

γ-H2AX作为标志物采用细胞内蛋白质印迹法（ICW）定量HeLa细胞中Colibactin相关基因毒性

Sophie Tronnet and Eric Oswald

2018年03月20日 7394 阅读

肠凝聚型大肠埃希杆菌对HEK293细胞的粘附测定

肠凝聚型大肠埃希杆菌对HEK293细胞的粘附测定

Jorge Luis Ayala-Lujan and Fernando Ruiz-Perez

2018年04月20日 6152 阅读

研究酗酒酒精毒性对小鼠肺泡巨噬细胞影响的细胞内侵袭和杀伤试验

研究酗酒酒精毒性对小鼠肺泡巨噬细胞影响的细胞内侵袭和杀伤试验

Victor Jimenez Jr and Fernando P Monroy

2019年01月20日 5944 阅读

Abstract

Moraxella catarrhalis is a human-restricted pathogen that is responsible for respiratory tract infections such as childhood otitis media (OM) and exacerbation of chronic obstructive pulmonary disease (COPD) in adults. Successful colonization and infection by M. catarrhalis depends on its ability to attach to the respiratory tract mucosal epithelium. This protocol describes a method to measure adherence of M. catarrhalis to epithelial cell lines in vitro.

Keywords: Moraxella catarrhalis (卡他莫拉菌) search Epithelial cells (上皮细胞) search Adhesion (粘附) search Respiratory tract (呼吸道) search Pathogenesis (发病机制) search

Materials and Reagents

  1. Human pharyngeal epithelial cell line Detroit 562 (ATCC, catalog number: CCL-138 )
  2. Type II alveolar epithelial cell line A549 (ATCC, catalog number: CCL-185 )
  3. DMEM + GlutaMAXTM-I (Life Technologies, Invitrogen™, catalog number: 31966-047 )
  4. Fetal calf serum (FCS) (Greiner Bio-One GmbH, catalog number: 758093S5403 )
  5. Trypsin-EDTA (0.25%-1 mM) (Life Technologies, catalog number: 25300-054 )
  6. Brain heart infusion (BHI) (Becton Dickinson and Company, catalog number: 237500 ) broth and BHI agar plates
  7. Antibiotics: spectinomycin or kanamycin (Merck KgaA, Calbiochem, catalog numbers: 567570-10 and 420311-5 )
  8. Bovine skin gelatin (Sigma-Aldrich, catalog number: G9382-100G )
  9. Glycerol (Merck KgaA, catalog number: 1.04092.1000)
  10. PBS without Ca2+ and Mg2+ (Westburg BV, catalog number: BE17-516F/12 )
  11. Saponin (Sigma-Aldrich, catalog number: 47036-50G-F )

Equipment

  1. 24-well tissue culture plate (Falcon®, catalog number: 353407 )
  2. 75 cm2 culture flask
  3. Centrifuge
  4. CO2 incubator
  5. Benchtop Incubator Shaker

Procedure

English
中文翻译

文章信息

版权信息

© 2013 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Vries, S. P. D. and Bootsma, H. J. (2013). Adhesion of Moraxella catarrhalis to Respiratory Tract Epithelial Cells. Bio-protocol 3(21): e956. DOI: 10.21769/BioProtoc.956.

ris ris Download Citation in RIS Format

分类

微生物学 > 微生物-宿主相互作用 > 体外实验模型

细胞生物学 > 细胞结构 > 细胞粘附

您对这篇实验方法有问题吗？

在此处发布您的问题，我们将邀请本文作者来回答。同时，我们会将您的问题发布到Bio-protocol Exchange，以便寻求社区成员的帮助。

0/150

tip 提问指南

+ 问题描述

写下详细的问题描述，包括所有有助于他人回答您问题的信息（例如实验过程、条件和相关图像等）。

post 发布问题
0 Q&A
pen 提交稿件