Dissection of Different Areas from Mouse Hippocampus

Materials and Reagents

1. Microspatula (Thermo Fisher Scientific, catalog number: 21-401-10 )
   
2. Short microspatula (Thermo Fisher Scientific, catalog number: 21-401-15 )
   
3. Filter paper (11 cm) (Thermo Fisher Scientific, catalog number: 09-795D )
   
4. WypAll or KimWipes
   
5. Ice buckets
   
6. Cloth diapers
   
7. Dry Ice
   
8. Biohazard waste bags
   
9. Adult laboratory mice
   
10. 10x Stock Cutting Solution (see Recipes)
    
11. 1 M KCl (see Recipes)
    
12. 1 M MgCl₂ (see Recipes)
    
13. 1 M CaCl₂ (see Recipes)
    
14. 1x Cutting Solution (make from 10x stock) (see Recipes)

Equipment

1. Surgical Scissors (Fine Science Tools, catalog number: 14130-17 )
   
2. Fine Scissors (Fine Science Tools, catalog number: 14094-11 )
   
3. Forceps (Fine Science Tools, catalog number: 11506-12 )
   
4. Scalpel handle (Fine Science Tools, catalog number: 10003-12 )
   
5. Scalpel blades #15 (Fine Science Tools, catalog number: 10015-00 )
   
6. Single edge razor blades
   
7. Petri dishes (100 mm x 15 mm) (Sigma-Aldrich, catalog number: CLS3160101 )
   
8. Transfer pipettes (Thermo Fisher Scientific, catalog number: 13-711-7M )
   
9. 20 ml beaker (Thermo Fisher Scientific, catalog number: 02-539-1 )
   
10. 600 ml beaker (Sigma-Aldrich, catalog number: CLS1000600 )
    
11. 1 L bottle (Sigma-Aldrich, catalog number: CLS13951L )
    
12. Nuclease-free microtubes (1.5-1.7 ml)
    
13. Plastic spoon
    
14. Microscope
    
15. Pressurized oxygen tank (95% O₂/5% CO₂) with tubing
    

Procedure