Dissection of Different Areas from Mouse Hippocampus
小鼠海马体不同部位的解剖
发布: 2013年11月05日第3卷第21期 DOI: 10.21769/BioProtoc.955 浏览次数: 27776
评审: Xuecai Ge
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参见作者原研究论文
The authors used this protocol in:
Nov 2012
Abstract
The hippocampus modulates a number of modules including memory consolidation, spatial navigation, temporal processing and emotion. A banana-shaped structure, the hippocampus is constituted of morphologically distinct subregions including the dentate gyrus, CA3 and CA1 (here, we do not distinguish the “hippocampus proper” which consists only of CA1, CA3 and smaller CA2 and CA4 areas, from the “hippocampal formation,” composed of these in addition to the dentate gyrus and subiculum). Distinct cell types give rise to unique axonal fiber pathways in the dentate gyrus, CA3 and CA1 subregions; accordingly, these areas may exhibit differential molecular profiles in response to a number of behavioral paradigms and pharmacological and genetic treatments. It is therefore in the interest of the investigator to dissect a specific subregion from the whole hippocampus. Here we outline a protocol for subregion-specific dissection from the adult mouse.
Memory (记忆)
Learning (学习)
CA1 (CA1)
Materials and Reagents
- Microspatula (Thermo Fisher Scientific, catalog number: 21-401-10 )
- Short microspatula (Thermo Fisher Scientific, catalog number: 21-401-15 )
- Filter paper (11 cm) (Thermo Fisher Scientific, catalog number: 09-795D )
- WypAll or KimWipes
- Ice buckets
- Cloth diapers
- Dry Ice
- Biohazard waste bags
- Adult laboratory mice
- 10x Stock Cutting Solution (see Recipes)
- 1 M KCl (see Recipes)
- 1 M MgCl2 (see Recipes)
- 1 M CaCl2 (see Recipes)
- 1x Cutting Solution (make from 10x stock) (see Recipes)
Equipment
- Surgical Scissors (Fine Science Tools, catalog number: 14130-17 )
- Fine Scissors (Fine Science Tools, catalog number: 14094-11 )
- Forceps (Fine Science Tools, catalog number: 11506-12 )
- Scalpel handle (Fine Science Tools, catalog number: 10003-12 )
- Scalpel blades #15 (Fine Science Tools, catalog number: 10015-00 )
- Single edge razor blades
- Petri dishes (100 mm x 15 mm) (Sigma-Aldrich, catalog number: CLS3160101 )
- Transfer pipettes (Thermo Fisher Scientific, catalog number: 13-711-7M )
- 20 ml beaker (Thermo Fisher Scientific, catalog number: 02-539-1 )
- 600 ml beaker (Sigma-Aldrich, catalog number: CLS1000600 )
- 1 L bottle (Sigma-Aldrich, catalog number: CLS13951L )
- Nuclease-free microtubes (1.5-1.7 ml)
- Plastic spoon
- Microscope
- Pressurized oxygen tank (95% O2/5% CO2) with tubing
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
- Sultan, F. A. (2013). Dissection of Different Areas from Mouse Hippocampus. Bio-protocol 3(21): e955. DOI: 10.21769/BioProtoc.955.
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Sultan, F. A., Wang, J., Tront, J., Liebermann, D. A. and Sweatt, J. D. (2012). Genetic deletion of Gadd45b, a regulator of active DNA demethylation, enhances long-term memory and synaptic plasticity. J Neurosci 32(48): 17059-17066.
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分类
神经科学 > 行为神经科学 > 认知
细胞生物学 > 组织分析 > 组织分离
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