Materials and Reagents

1. Sterile plastic bags, 30 g capacity (~30 ml) (Whirl-Pak^® Write-On Bags) (Nasco, catalog number: B01067WA )
   
2. 70% Ethanol (Merck KgaA, Emsure^®, catalog number: 1009834000 )
   
3. Latex examination gloves (Dermagrip^®, catalog number: D1402-14 )
   
4. Pyrogen-free Type 1+ grade distilled water (Milli-Q Direct 8 and 16 Ultrapure Water System)
   
5. Dulbecco′s Phosphate Buffered Saline (Gibco, catalog number: 21300-058 )
   
6. Tween^® 20 (Sigma-Aldrich, catalog number: P5927 )
   
7. Tryptone Soya Agar (Oxoid Limited, catalog number: CM0131 ) (see Recipes), or other media according to culture bacterial requirements
   
8. PBS 0.1% Tween 20 (see Recipes)
   

Equipment

1. 24-well plates, low binding cell (Sigma-Aldrich, catalog number: Z721077 )
   
2. Beaker (100 ml)
   
3. Glass alcohol burner (WHEATON, catalog number: WHE-237070 )
   
4. Surgical instruments for mice (Mouse Surgical Kit) (Kent Scientific, model: INSMOUSEKIT )
   
5. Drigalski spatula (Thermo Fisher Scientific, catalog number: NC0242405 )
   
6. Micropipettes (10-100 μl and 100-1,000 μl)
   
7. Micropipette tips (100 μl and 1 ml)
   
8. 1.5 ml eppendorf tubes (Eppendorf, catalog number: 0 22363204 )
   
9. Tuberculin plastic syringe (1 ml) with 25-27 g needle gauge
   
10. Plastic petri dishes (90 x 15 mm)
    
11. Disposable medical plastic gloves (Lab Depot, catalog number: 266-P )
    
12. Small sharp scissors  and forceps
    
13. Water purification system (Milli-Q Direct 8 and 16 Ultrapure Water System) (EMD Millipore, model: ZR0Q00800 )
    
14. Bacterial incubator (Forma^® Direct Heat CO₂ Incubator) (Thermo Fisher Scientific, model: 310 )
    
15. Autoclave (Yamato Scientific, model: SQ-500C )
    
16. Analytical balance electronic digital (± 0.0001 g)
    
17. Laminar flow cabinet (Esco Global, Labculture^® Class II, Type A2, model: LA2-3A2 )

Procedure