Ovule Clearing Method for Solanaceous Species
茄属胚珠整体透明技术
发布: 2013年11月05日第3卷第21期 DOI: 10.21769/BioProtoc.951 浏览次数: 11235
评审: Tie Liu
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参见作者原研究论文
The authors used this protocol in:
Mar 2013
Abstract
Depending on the species, embryo sacs can be difficult to observe. Ovule clearing allows precise observation of whole tissues under Differential interference contrast (DIC) microscopy. The use of Methyl Salicylate as a clearing agent has proved to be particularly reliable for Solanaceous species.
Ovule (胚珠)
Reproduction (繁殖)
Embryo sac (胚囊)
Differential interference contrast (DIC) (微分干涉对比（DIC）)
Materials and Reagents
- Flower buds
- 37% Formaldehyde
- Glacial acetic acid
- Methyl Salicylate
- Ethanol (≥ 95%)
- Fixative solution (FAA) (see Recipes)
- Clearing solution (see Recipes)
Equipment
- Forceps
- Microscope with differential interference contrast (DIC)
- Printed coated microscopy slides with wells (to avoid crushing the tissues)
- Cover slips
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Loubert-Hudon, A. and Matton, D. P. (2013). Ovule Clearing Method for Solanaceous Species. Bio-protocol 3(21): e951. DOI: 10.21769/BioProtoc.951.
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分类
植物科学 > 植物细胞生物学 > 细胞染色
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