Xenograft Tumor Growth Assay
异种移植肿瘤增生分析
发布: 2013年10月20日第3卷第20期 DOI: 10.21769/BioProtoc.948 浏览次数: 14121
评审: Lin FangPinchas Tsukerman Fanglian He
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参见作者原研究论文
The authors used this protocol in:
Jan 2013
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Abstract
Chronic inflammation drives initiation of hepatocellular carcinoma (HCC), but the underlying mechanisms linking inflammation and tumor formation remain obscure. In this study, Xenograft tumor assay was used to determine the tumorigenic activity of hepatoma cells with ISX over expression on nude mice in vivo.
Materials and Reagents
- HCC cells (Hep G2: ATCC® HB-8065 TM and Hep 3B: ATCC® HB-8064 TM)
- CAnN.Cg-Foxn1nu/CrlBltw Nude mice
- ISX fused with GFP or ISX shRNAi expression constructs
- PBS (MDBio)
- 2.5% Trypsin (10x) (Gibco , catalog number: 15090-046 )
- MEM Culture medium (Gibco, catalog number: 61100-061 )
Equipment
- 100 mm2 plate
- 15 ml conical tubes
- Centrifuge (Beckman Coulter)
- Eppendorf
- 1 ml syringes (0.45 x 13 mm)
- Tissue culture hood
- Caliper (Digital Caliper)
Procedure
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文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Wang, L. and Hsu, S. (2013). Xenograft Tumor Growth Assay. Bio-protocol 3(20): e948. DOI: 10.21769/BioProtoc.948.
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分类
癌症生物学 > 增殖信号转导 > 动物模型 > 增殖分析
癌症生物学 > 炎症 > 肿瘤形成 > 细胞侵袭
生物化学 > 蛋白质 > 表达
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