Hypertrophy Analysis and Quantification in Embryonic Cardiomyocites
胚胎心肌细胞的肥大分析和量化
发布: 2013年10月20日第3卷第20期 DOI: 10.21769/BioProtoc.938 浏览次数: 10012
评审: Fanglian HeLin FangAnonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Feb 2013
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Abstract
Myocardial growth goes from proliferation to hypertrophy during development. The measurement of the relative cell area provides information of cardiomyocyte hypertrophy, which is ideal for studying myocardial development.
Materials and Reagents
- Mouse embryos
- 4-6 μm paraffin sections of embryonic hearts
- 4% PFA
- Paraffin
- Xylene (Merck KGaA, catalog number: 8.08691.1000 )
- Ethanol (Merck KGaA, catalog number: 1.00983.1000 )
- PBS
- Wheat germ agglutinin (WGA) conjugates tetramethylrhodamine (Life Technologies, Molecular Probes®, catalog number: W849 ) (1:100)
- DAPI (Invitrogen, catalog number: D1306 )
- Distilled water
- Fluorescence mounting media (Fluoromont-G) (SouthernBiotech, catalog number: 0100-01 )
Equipment
- Microtome
- Fluorescence microscope
- Humid chamber
Software
- Image analysis software (Image J)
Procedure
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文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Luxán, G. and Pompa, J. L. D. L. (2013). Hypertrophy Analysis and Quantification in Embryonic Cardiomyocites. Bio-protocol 3(20): e938. DOI: 10.21769/BioProtoc.938.
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分类
细胞生物学 > 细胞成像 > 荧光
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