Fluorescence Measurement of Postharvest Physiological Deterioration (PPD) in Cassava Storage Roots
荧光测定木薯储藏根采后的生理劣变（PPD）
发布: 2013年09月20日第3卷第18期 DOI: 10.21769/BioProtoc.909 浏览次数: 13127
评审: Tie Liu
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参见作者原研究论文
The authors used this protocol in:
Mar 2013
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Abstract
Cassava (Manihot esculenta Crantz) is a perennial root crop in the tropics. Within 24-72 hours of harvest the storage roots deteriorate rapidly, thereby necessitating their prompt processing or consumption. Postharvest physiological deterioration (PPD) of cassava storage roots is the result of a rapid oxidative burst, which leads to discoloration of the vascular tissues. The various fluorogenic probes available for in vivo reactive oxygen species (ROS) imaging could reveal complex spatial and temporal dynamics in plant tissues. Fluorescence measurement of PPD became widely used assay for ROS. Most of the ROS probes passively diffuse across cell membranes localize in the mitochondria, and exhibit fluorescence. Due to its high sensitivity to ROS and ease of loading and detection, the Dihydrorhodamine123 probe has been widely used in plants to monitor ROS accumulation in response to various stimuli and range of developmental processes.
Keywords: Cassava (木薯)
Storage root (贮藏根)
Postharvest physiological deterioration (采后生理恶化)
Fluorescence (荧光)
Materials and Reagents
- DMSO (Sangon Biotech, catalog number: D0231 )
- Dihydrorhodamine123 (Invitrogen, Molecular Probes®, catalog number: D632 ) (see Recipes)
- MitoTracker Deep Red FM (Invitrogen, Molecular Probes®, catalog number: M22426 ) (see Recipes)
- 0.1 M sodium phosphate buffer (pH 7.0) (see Recipes)
Equipment
- 48-well plate
- Razor blade
- Slides
- Centrifuge
- Confocal laser scanning microscope (Zeiss, model: LSM 510 META )
Procedure
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文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
- Xu, J., Fellman, J. K. and Zhang, P. (2013). Fluorescence Measurement of Postharvest Physiological Deterioration (PPD) in Cassava Storage Roots. Bio-protocol 3(18): e909. DOI: 10.21769/BioProtoc.909.
- Xu, J., Duan, X., Yang, J., Beeching, J. R. and Zhang, P. (2013). Enhanced reactive oxygen species scavenging by overproduction of superoxide dismutase and catalase delays postharvest physiological deterioration of cassava storage roots. Plant Physiol 161(3): 1517-1528.
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分类
植物科学 > 植物生理学 > 非生物胁迫
生物化学 > 其它化合物 > 活性氧
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