Immunoprecipitation of ROR1
ROR1的免疫共沉淀
发布: 2013年09月20日第3卷第18期 DOI: 10.21769/BioProtoc.904 浏览次数: 7965
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参见作者原研究论文
The authors used this protocol in:
Nov 2012
Abstract
ROR1 is a receptor tyrosine kinase family member studied for its roles in development and cancer. Here we describe a protocol for immunoprecipitation of endogenous ROR1 from t(1;19) (a disease subtype categorized by its chromosome translocation) acute lymphoblastic leukemia immortalized cell lines.
Materials and Reagents
- ROR1-positive cells (e.g. RCH-ACV cells, Kasumi-2 cells, 697 cells)
- Cell lysis buffer (Cell Signaling Technology, catalog number: 9803 )
- Protease inhibitor cocktail (cOmplete Mini EDTA-free) (Roche, catalog number: 04693159001 )
- Phosphatase inhibitor cocktail II (Sigma-Aldrich, catalog number: P5726 )
- Antibody specific for ROR1 (R&D Systems, catalog number: AF2000 )
- Isotype matched control (R&D Systems, catalog number: AB-108-C )
- Protein G Agarose beads (EMD Millipore, catalog number: 16-266 )
- SDS
- BSA
- DTT
Equipment
- P1000 pipette
- Centrifuge
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Bicocca, V. T. and Tyner, J. W. (2013). Immunoprecipitation of ROR1. Bio-protocol 3(18): e904. DOI: 10.21769/BioProtoc.904.
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分类
生物化学 > 蛋白质 > 免疫检测
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