Mouse Macrophage Differentiation by Induction with Macrophage Colony-Stimulating Factor
通过用巨噬细胞集落刺激因子（M-CSF)诱导小鼠巨噬细胞分化
发布: 2013年09月05日第3卷第17期 DOI: 10.21769/BioProtoc.893 浏览次数: 13661
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参见作者原研究论文
The authors used this protocol in:
Jan 2013
Abstract
Macrophages are differentiated from circulating blood monocytes and act as tissue-resident professional phagocytes. Macrophages function in both innate and adaptive immune systems of vertebrate animals. The cytokine macrophage colony-stimulating factor (M-CSF) is essential for the proliferation and differentiation of monocytes. Here, we described a simple method to induce the differentiation of mouse bone marrow-derived myeloid precusor cells into macrophages in the presence of M-CSF.
Keywords: Monocytes (单核细胞)
Differentiation (区别)
Macrophages (巨噬细胞)
Materials and Reagents
- Mice of interest
- 1x DPBS (Lonza, catalog number: 17-512F )
- ACK lysing buffer (Lonza, catalog number: 10-548E )
- Dulbecco’s Modified Eagle’s Medium with high glucose and without L-glutamine (DMEM) (Lonza, catalog number: 12-614F )
- Fetal Calf Serum (FCS) (Thermo Scientific, catalog number: SH30070.03 )
- Murine M-CSF (Peprotech, catalog number: 315-02 )
- 100x Penicillin/Streptomycin (Invitrogen, catalog number: 10378-016 )
- Complete DMEM medium (see Recipes)
Equipment
- 40 μm nylon strainer (BD Falcon, catalog number: 352340 )
- Needle (Gauge #25)
- Centrifuge
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Liu, Y., Chen, K., Wang, C., Gong, W., Yoshimura, T., Wang, J. M. and Liu, M. (2013). Mouse Macrophage Differentiation by Induction with Macrophage Colony-Stimulating Factor . Bio-protocol 3(17): e893. DOI: 10.21769/BioProtoc.893.
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分类
免疫学 > 免疫细胞分离 > 维持和分化
细胞生物学 > 细胞分离和培养 > 细胞分离
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