Figure 1. 3 dpi Arabidopsis gall from M. javanica. Blue lines indicate the collected material, galls with a small portion of roots (that allows easy handling), frozen and processed for RNA extraction.

Materials and Reagents

1. Arabidopsis infected by M. javanica (3 dpi or 7 dpi)
   
2. TRI Reagent (Molecular Research Centre, MRC, catalog number: TR-118 )
   
3. Chloroform (Scharlau, catalog number: CL02031000 )
   
4. Isopropanol (Merck KGaA, catalog number: 0 9634 )
   
5. Sodium citrate (Sigma-Aldrich, catalog number: s4641 )
   
6. Sodium chloride (Duchefa, catalog number: S0520 )
   
7. NaOH (Duchefa, catalog number: s0523 )
   
8. Ethanol (Merck KGaA, catalog number: 100986 )
   
9. Diethylpyrocarbonate (DEPC) (Sigma-Aldrich, catalog number: D5758 )
   
10. RNase-free water
    
11. Acetone (Thermo Fisher Scientific, catalog number: A/0600/17 )
    
12. Liquid nitrogen
    
13. RNeasy Mini Kit (QIAGEN, catalog number: 74104 ))
    
14. RNase-Free DNase Set (QIAGEN, catalog number: 79254 )
    
15. High salt precipitation solution (see Recipes)
    

Equipment

1. Centrifuges
   
2. Microfuge
   
3. 1.5 ml Eppendorf^® tubes
   
4. Porcelain mortar and pestles, textured surface on bowl interior, 60 mm diameter
   
5. Fume hood
   

Procedure