水稻NO3-吸收量的测定-SITE法（scanning ion-electrode technique)

Zhong  Tang; Guohua  Xu

doi:10.21769/BioProtoc.876

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Peer-reviewed

Measurement of Net NO₃^- Flux in Rice Plants with the SIET System

水稻NO3-吸收量的测定-SITE法（scanning ion-electrode technique)

Zhong Tang

Guohua Xu email

发布: 2013年08月20日第3卷第16期 DOI: 10.21769/BioProtoc.876 浏览次数: 8581

评审: Tie Liu

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参见作者原研究论文

The authors used this protocol in:

Cover of Plant Physiology, featuring study using the protocol.

Dec 2012

Special Issue on Protocols for Organoid Research

实验方案合集

Cell Imaging - A Special Collection for Cell Bio 2023

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Abstract

SIET (scanning ion-electrode technique) is a new technique to study the flow rate of the ions and molecules in real time in living biomaterials by using microelectrodes and microsensor. This technique allows non-invasive, simultaneous measurement of fluxes of specific ions at the surface of an intact plant. It has high temporal and spatial resolutions. This protocol uses the SIET system for the measurement of ions flux rate in rice plants.

Keywords: Nitrate transporter (硝酸盐转运蛋白)

Materials and Reagents

Rice seedlings: Three weeks old seedlings
Plastic supporting netting
Tributylchlorosilane (Fluka, catalog number: 90796 )
NH₄NO₃
KH₂PO₄
K₂SO₄
CaCl₂^.2H₂O
MgSO₄^.7H₂O
Na₂SiO₃
NaFeEDTA
H₃BO₃
MnCl₂^.4H₂O
CuSO₄^.5H₂O
ZnSO₄^.7H₂O
Na₂MoO_4^.2H₂O
International Rice Research Institute (IRRI) nutrient solution (see Recipes)
Calibrate solution (see Recipes)
Measuring solution (see Recipes)

Equipment

Small plastic dish (6 cm diameter)
Measuring chamber
Ion-selective electrodes (Clark Electromedical, model: GC150-10 )
BIO-IM (NMT-YG-100, Younger USA LLC, Amherst, model: MA01002 ) with ASET 2.0

Software

ASET 2.0 (Sciencewares, Falmouth, catalog number: MA 02540)
iFluxes 1.0 (YoungerUSA, LLC, Amherst, catalog number: MA 01002) software

Procedure

English

中文翻译

文章信息

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如何引用

Readers should cite both the Bio-protocol article and the original research article where this protocol was used:

Tang, Z. and Xu, G. (2013). Measurement of Net NO₃^- Flux in Rice Plants with the SIET System. Bio-protocol 3(16): e876. DOI: 10.21769/BioProtoc.876.
Tang, Z., Fan, X., Li, Q., Feng, H., Miller, A. J., Shen, Q. and Xu, G. (2012). Knockdown of a rice stelar nitrate transporter alters long-distance translocation but not root influx. Plant Physiol 160(4): 2052-2063.