15N 标记法测定水稻幼苗中硝酸盐的吸收和积累

Zhong  Tang; Guohua  Xu

doi:10.21769/BioProtoc.875

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Peer-reviewed

Determination of Nitrate Uptake and Accumulation Using ¹⁵N in Rice Seedlings

15N 标记法测定水稻幼苗中硝酸盐的吸收和积累

Zhong Tang

Guohua Xu email

发布: 2013年08月20日第3卷第16期 DOI: 10.21769/BioProtoc.875 浏览次数: 8240

评审: Tie Liu

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参见作者原研究论文

The authors used this protocol in:

Cover of Plant Physiology, featuring study using the protocol.

Dec 2012

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Abstract

Nitrogen-15 is a rare stable isotope of nitrogen. This isotope is often used in agricultural research. For example, Nitrogen-15 is used to trace mineral nitrogen compounds and translocate the nitrogen molecule in plants. This protocol is used to determine nitrate uptake and accumulation in rice seedlings by using Nitrogen-15.

Materials and Reagents

Rice seedlings: Four weeks old seedlings
NH₄NO₃ (catalog number: 6484-52-2 )
KH₂PO₄ (catalog number: 7778-77-0 )
K₂SO₄ (catalog number: 7778-80-5 )
CaCl₂^.2H₂O (catalog number: 94248-52-9)
MgSO₄^.7H₂O (catalog number: 10034-99-8 )
Na₂SiO₃ (catalog number: 1344-09-8 )
NaFeEDTA (catalog number: 7720-78-7; 139-33-3)
H₃BO₃ (catalog number: 10043-35-3 )
MnCl₂^.4H₂O (catalog number: 20603-88-7)
CuSO₄^.5H₂O (catalog number: 7758-99-8 )
ZnSO₄^.7H₂O (catalog number: 7446-20-0 )
Na₂MoO₄^.2H₂O (catalog number: 7631-95-0 )
CaSO₄^.2H₂O (catalog number: 10101-41-4 )
IRRI nutrient solution (see Recipes)
0.1 mM CaSO₄ solution (see Recipes)
K¹⁵NO₃(catalog number: 57654-83-8 ) (see Recipes)

Equipment

Isotope Ratio Mass Spectrometer (Thermo Fisher Scientific, model: MAT253 )
Elemental Analyzer (Thermo Fisher Scientific, model: Flash EA1112 HT )

Procedure

English

中文翻译

文章信息

版权信息

如何引用

Readers should cite both the Bio-protocol article and the original research article where this protocol was used:

Tang, Z. and Xu, G. (2013). Determination of Nitrate Uptake and Accumulation Using ¹⁵N in Rice Seedlings. Bio-protocol 3(16): e875. DOI: 10.21769/BioProtoc.875.
Tang, Z., Fan, X., Li, Q., Feng, H., Miller, A. J., Shen, Q. and Xu, G. (2012). Knockdown of a rice stelar nitrate transporter alters long-distance translocation but not root influx. Plant Physiol 160(4): 2052-2063.