Cellular Extract Preparation for Superoxide Dismutase (SOD) Activity Assay
用于超氧化物歧化酶（SOD）活性试验的拟南芥细胞提取物的制备
发布: 2013年07月05日第3卷第13期 DOI: 10.21769/BioProtoc.811 浏览次数: 26187
评审: Tie Liu
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参见作者原研究论文
The authors used this protocol in:
Jan 2013
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Abstract
Superoxide dismutase (SOD) acts as a primary defence against reactive oxygen species (ROS) by converting O2- to O2 and H2O2. Members of this enzyme family include CuZnSOD, MnSOD and FeSOD. Most eukaryotes harbor CuZnSOD and MnSOD, and FeSOD is found in plants and prokaryotes. This protocol is to demonstrate how to prepare the cellular extract for the identification and characterization of SODs in planta.
Keywords: SOD (草地)
Activity Assay (活性测定)
N (n)
Materials and Reagents
- Nitroblue tetrazolium (NBT) (Sigma-Aldrich, catalog number: N6876 )
- Riboflavin (Sigma-Aldrich, catalog number: R4500 )
- N,N,N’,N’-Tetramethylethylenediamine (TEMED) (Sigma-Aldrich, catalog number: T9281 )
- KCN (Sigma-Aldrich, catalog number: 60178 )
- H2O2 (Sigma-Aldrich, catalog number: 349887 )
- NBT solution (see Recipes)
- Grinding buffer (see Recipes)
- Riboflavin solution (see Recipes)
- KCN solution (see Recipes)
- H2O2 solution (see Recipes)
Equipment
- A light box (white light)
- Centrifuge (Heraecus, Biofuge fresco)
- Protein gel cassette
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Kuo, W., Huang, C., Shih, C. and Jinn, T. (2013). Cellular Extract Preparation for Superoxide Dismutase (SOD) Activity Assay. Bio-protocol 3(13): e811. DOI: 10.21769/BioProtoc.811.
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分类
植物科学 > 植物生物化学 > 蛋白质 > 分离和纯化
生物化学 > 蛋白质 > 活性
生物化学 > 其它化合物 > 活性氧
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