Materials and Reagents

1. Indole derivative:
   TRP (Sigma-Aldrich, catalog number: T0254-500g )
   IAD (indole-3-acetaldehyde) (Sigma-Aldrich, catalog number: I1000-100mg )
   IAA (Sigma-Aldrich, catalog number: I5148-2g )
   
2. Standard microscope coverslips
   
3. Standard microscope slides
   
4. 0.002% (v/v) Tween water 20
   
5. 0.9% NaCl
   
6. FeCl₃
   
7. HClO₄
   
8. NaNO₃
   
9. MgSO₄^.7H₂O
   
10. KH₂PO₄
    
11. Orthophosphoric acid
    
12. Glucose
    
13. Peptone
    
14. Yeast extract
    
15. Casamine acids
    
16. Microelements
    
17. Agar
    
18. Tryptophan (Sigma-Aldrich, catalog number: T0254-500g)
    
19. Ethyl acetate
    
20. Aluminum foil
    
21. p-dimethylaminobenzaldehyde (Sigma-Aldrich)
    
22. Van Urk reagent (see Recipes)
    
23. Salkowski test reagents (see Recipes)
    
24. 10 mM orthophosphoric acid (see Recipes)
    
25. Complete medium (see Recipes)
    
26. 20x salt solution (see Recipes)
    
27. Microelements (see Recipes)
    

Equipment

1. Erlenmeyer flasks (100 ml)
2. Neubauer improved counting chamber (Marienfeld-Superior, Lauda, Königshofen, Germany)
   
3. Scalpel (sterile)
   
4. Whatman paper
   
5. Miracloth filter 15 cm x 15 cm (Merck KGaA, catalog number: 475855 )
   
6. Drigalski spatula (sterile)
   
7. Disposable cuvettes
   
8. Preval sprayer
   
9. Thin layer chromatography (TLC) chamber
   
10. Petri dishes (12 mm in diameter)
    
11. 15 ml sterile Falcon tubes
    
12. 2 ml Eppendorf tubes
    
13. Glass test tubes
    
14. Incubator e.g. Certomat BS-1 (B. Braun Biotech International)
    
15. Speedvac sc110 (Savant, Thermo Fisher Scientific)
    
16. Biofuge Primo R (Heraeus) (Rotor, catalog number: 7590 )
    
17. Heraeus Pico 17 centrifuge (Heraeus)
    
18. Spectrophotometer e.g. Ultrospec 3,000 proUV/Visible (GE Healthcare)
    
19. Clean bench Hera Safe (Heraeus)
    
20. Hybridization oven (Hybaid Shake n’ Stack, Thermo Fisher Scientific)
    

Procedure