小鼠主嗅上皮中的嗅电图(EOG)记录

Xuanmao Chen; Zhengui Xia; Daniel R. Storm

doi:10.21769/BioProtoc.789

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Peer-reviewed

Electroolfactogram (EOG) Recording in the Mouse Main Olfactory Epithelium

小鼠主嗅上皮中的嗅电图(EOG)记录

Xuanmao Chen

ZX Zhengui Xia

Daniel R. Storm email

发布: 2013年06月05日第3卷第11期 DOI: 10.21769/BioProtoc.789 浏览次数: 9832

评审: Xuecai Ge

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参见作者原研究论文

The authors used this protocol in:

Cover of The Journal of Neuroscience, featuring study using the protocol.

Nov 2012

Special Issue on Protocols for Organoid Research

实验方案合集

Cell Imaging - A Special Collection for Cell Bio 2023

Abstract

Olfactory sensory neurons in the main olfactory epithelium (MOE) are responsible for detecting odorants and EOG recording is a reliable approach to analyze the peripheral olfactory function. However, recently we revealed that rodent MOE can also detect the air pressure caused by airflow. The sensation of airflow pressure and odorants may function in synergy to facilitate odorant perception during sniffing. We have reported that the pressure-sensitive response in the MOE can also be assayed by EOG recording. Here we describe procedures for pressure-sensitive as well as odorant-stimulated EOG measurement in the mouse MOE. The major difference between the pressure-sensitive EOG response and the odorant-stimulated response was whether to use pure air puff or use an odorized air puff.

Keywords: Olfaction (嗅觉)

Materials and Reagents

3-heptanone (Sigma-Aldrich)
Forskolin (Sigma-Aldrich)
IBMX (3-isobutyl-1-methylxanthine) (Sigma-Aldrich)
SCH202676 (Sigma-Aldrich)
Compressed pure nitrogen air (Praxair Inc)
Thin-wall glass capillary (OD 1.0 mm ID 0.78 mm) (Harvard Apparatus)
C57Bl/6 mice (Charles River or Jackson Lab)
Note: Mice used were 2.5-5 months age-matched males or females. Mice were maintained on a 12 h light/dark cycle at 22 °C, and had access to food and water ad libitum. All animal procedures were approved by the Institutional Animal Care and Use Committee at the University of Washington and performed in accordance with their guidelines.
Ringer’s solution (see Recipes)