Insulin Tolerance Test and Hyperinsulinemic-euglycemic Clamp
胰岛素耐受试验和高胰岛素-正血糖钳夹试验
发布: 2013年05月20日第3卷第10期 DOI: 10.21769/BioProtoc.776 浏览次数: 11388
评审: Anonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Dec 2012
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Abstract
The two tests are used to evaluate in vivo sensitivity to insulin in mouse. The hypoerinsulinemic-euglycemic clamp provides information about the sensitivity to insulin in liver and other metabolically relevant tissues.
Keywords: Insulin (胰岛素)
Diabetes (型糖尿病)
Mouse (小鼠)
Glucose (葡萄糖)
Materials and Reagents
- Human insulin (Eli Lilly, Indianapolis, IN)
- [3-3H] glucose (Perkin Elmer, catalog number: NET331A250UC )
- 2-deoxy-D-[1-14C] glucose (2-[14C]DG) (PerkinElmer, catalog number: NET328250UC )
Equipment
- Contour blood glucometer (Bayer)
Procedure
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文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Paschos, G. K. and FitzGerald, G. A. (2013). Insulin Tolerance Test and Hyperinsulinemic-euglycemic Clamp. Bio-protocol 3(10): e776. DOI: 10.21769/BioProtoc.776.
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分类
生物化学 > 蛋白质 > 活性
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