Improve Research Reproducibility A Bio-protocol resource
Peer-reviewed
tooltip

In vitro Lipid Transfer Assay

体外脂质转移试验

YZ Yan Zhang
XW Xiaochen Wang

发布: 2013年05月05日第3卷第9期 DOI: 10.21769/BioProtoc.691 浏览次数: 13300

download pdf PDF PDF
ask Q&A
引用
收藏
Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of Current Biology, featuring study using the protocol.

Jul 2012

Advertisement

Special Issue on Protocols for Organoid Research
Technologies for RNA Detection
Presubmission Inquiry

实验方案合集

专注于特定技术或应用的、详细的、经过同行评审的实验方案合集

Cancer Research
Immunology
Developmental Biology
Microbiology
Neuroscience
Cell Imaging - A Special Collection for Cell Bio 2023
查看更多 more

相关实验方案

秀丽隐杆线虫中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性测定实验方案

秀丽隐杆线虫中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性测定实验方案

Jing Zhang [...] Lili Xue

2017年08月20日 39539 阅读

采用液态稳定型CO₂检测试剂的偶联酶法测定细胞裂解液中鸟氨酸脱羧酶活性

采用液态稳定型CO₂检测试剂的偶联酶法测定细胞裂解液中鸟氨酸脱羧酶活性

Jung-Mao Hsu

2026年08月05日 215 阅读

基于组织的酸性神经酰胺酶活性荧光检测

基于组织的酸性神经酰胺酶活性荧光检测

Arielle Manabat [...] Laura Volpicelli-Daley

2026年08月20日 314 阅读

Abstract

This is a protocol to detect lipid transfer activity of NRF-5, a member of the LPS binding/lipid transfer protein family. The lipid transfer activity is examined by using isotope-labeled cholesterol and liposomes, and tested in two directions (Figure 1): from proteins to liposomes and from liposomes to proteins.

Materials and Reagents

  1. PC (Avanti-Polar Lipids)
  2. PE (Avanti-Polar Lipids)
  3. Cholesterol (Avanti-Polar Lipids, catalog number: 700000 )
  4. 1,2-Dioleoyl-sn-Glycero-3-Phosphoethanolamine (DOPE) (Avanti, catalog number: 850725 )
  5. 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine (DOPC) (Avanti, catalog number: 770375 )
  6. 1 mCi (37 MBq) (PerkinElmer,  catalog number: NET139001MC )
  7. Protein of interest tagged with Flag
  8. Tris-HCl (pH 7.4)
  9. NaCl
  10. Anti-Flag M2 agarose beads (Sigma-Aldrich, catalog number: A2220 )
  11. Flag peptide (Sigma-Aldrich, catalog number: F3290 )
  12. Chloroform
  13. Wash buffer (see Recipes)
  14. Elution buffer (see Recipes)

Equipment

  1. Avanti Mini-Extruder (Avanti, catalog number: 610023 )
  2. Vortexer
  3. Centrifuges
  4. Rotator
  5. Branson tip-sonicator (Cole-parmer Cp750)
  6. Scintillation counter (Wallac MicroBeta TriLux, catalog number: 1450-023 )

Procedure

English
中文翻译

文章信息

版权信息

© 2013 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Zhang, Y. and Wang, X. (2013). In vitro Lipid Transfer Assay. Bio-protocol 3(9): e691. DOI: 10.21769/BioProtoc.691.

ris ris Download Citation in RIS Format

分类

生物化学 > 蛋白质 > 活性

生物化学 > 脂质 > 脂质转运

您对这篇实验方案有问题吗？

在此处发布您的问题，我们将邀请本文作者来回答。同时，我们会将您的问题发布到Bio-protocol Exchange，以便寻求社区成员的帮助。

post 发布问题
0 Q&A
pen 提交稿件