利用卵清蛋白刺激构建致敏模型

François Daubeuf; Laurent Reber; Nelly Frossard

doi:10.21769/BioProtoc.644

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Peer-reviewed

Allergen Sensitization and Challenge to Ovalbumin

利用卵清蛋白刺激构建致敏模型

FD François Daubeuf

LR Laurent Reber

NF Nelly Frossard email

发布: 2013年04月05日第3卷第7期 DOI: 10.21769/BioProtoc.644 浏览次数: 17304

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参见作者原研究论文

The authors used this protocol in:

Cover of The Journal of Immunology, featuring study using the protocol.

Apr 2012

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Abstract

This protocol describes the sensitization and challenge of mice with ovalbumin for use as an acute murine model of asthma. This protocol induces reproducible airway inflammation and remodelling, and bronchial hyperresponsiveness to methacholine as measured by barometric plethysmography, as well as by the Flexivent^® technique in Balb/c mice.

Keywords: Asthma (哮喘)

Allergen sensitization (过敏原)

Ovalbumin (卵清蛋白)

Intranasal challenge (鼻内挑战)

Mouse (小鼠)

Background

Materials and Reagents

Sterile saline (0.9% NaCl) (B. Braun)
Chicken egg albumin (ovalbumin, grade V) (Sigma-Aldrich, catalog number: A-5503 )
Aluminium hydroxide (Sigma-Aldrich, catalog number: 23918-6 )
Ketamine (Imalgene^®,Mérial)
Xylazine (2%) (Rompun^®, Bayer)
Ovalbumin (see Recipes)
Anaesthetics (see Recipes)

Equipment

"1 ml" sterile syringes (Terumo, catalog number: SS+01T1 )
"25 G" 0.5 mm sterile needles (Terumo, catalog number: NN-2516R )
Sterile tips (Starlab, catalog number: S1111-0800 )
Precision pipette (20 μl)
End-over-end rotator (SB2, Stuart)
15-ml Falcon tube

Procedure

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如何引用

Daubeuf, F., Reber, L. and Frossard, N. (2013). Allergen Sensitization and Challenge to Ovalbumin. Bio-protocol 3(7): e644. DOI: 10.21769/BioProtoc.644.