Primary Culture of Cortical Neurons
皮层神经元的初代培养
发布: 2013年04月20日第3卷第8期 DOI: 10.21769/BioProtoc.496 浏览次数: 27199
评审: Anonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Nov 2012
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Abstract
Primary culture of neurons from cerebral cortex is a popular model to study neuronal function in vitro and to explore the molecular mechanism of neurite outgrowth in the developing and adult central nervous system. This protocol is for preparing a culture of cerebral cortical neurons from postnatal rodent brain (Muramatsu et al., 2012). One day after cell plating, we can observe neurite outgrowth by microscope.
Keywords: Brain (脑)
Postnatal (产后)
Mouse (小鼠)
Vitro (体外)
Dissociate (游离)
Materials and Reagents
- Poly-L-lysine (Sigma-Aldrich, catalog number: P2636 )
- Dulbecco's Modified Eagle Medium, DMEM (Life Technologies, InvitrogenTM, catalog number: 12800-017 )
- Fetal bovine serum (Life Technologies, Gibco®, catalog number: 10437 )
- 2.5% Trypsin solution (Life Technologies, Gibco®, catalog number: 15090-046 )
- DNase (Sigma-Aldrich, catalog number: DN25 )
- Penicillin/streptomycin (Life Technologies, Gibco®, catalog number: 15140 )
- NaH2PO4.2H2O
- Na2HPO4.12H2O
- NaCl
- DMEM
- FBS
- Serum-containg culture medium (see Recipes)
- PBS (see Recipes)
Equipment
- Cell culture incubator
- Centrifuge
- Stereoscopic microscope
- Scissors, forceps, and knives
- Sterile filter (0.45 μm)
- Cell strainer 70 μm nylon (BD Biosciences, catalog number: 352350 )
- 24 well culture plate
Procedure
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文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Muramatsu, R. and Yamashita, T. (2013). Primary Culture of Cortical Neurons. Bio-protocol 3(8): e496. DOI: 10.21769/BioProtoc.496.
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分类
神经科学 > 发育 > 神经元
细胞生物学 > 细胞分离和培养 > 细胞分化
神经科学 > 神经解剖学和神经环路 > 动物模型
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