Fusarium Virulence Assay on Wheat and Barley Seedlings
小麦和大麦幼苗的抗镰刀菌毒性试验
发布: 2013年04月05日第3卷第7期 DOI: 10.21769/BioProtoc.446 浏览次数: 13716
评审: Fanglian He
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参见作者原研究论文
The authors used this protocol in:
Sep 2012
Abstract
Fusarium root and crown rot is a very important complex disease of small grain cereals worldwide which may lead to very high yield losses. Traditional virulence assays are time consuming and often require plants to be grown in greenhouses or climatic chambers in soil. We describe a rapid laboratory assay for assessing such a disease in wheat and barley seedlings. The method could be successfully used for testing fungal virulence as well as to assess plant resistance.
Background
Materials and Reagents
- Fungal strains
- 25% Campbell`s V8 juice (pH 6.5 with NaOH)
- 1.2% agar plates (9 cm diameter)
- Wheat and barley seeds
- Sterilizing solution (0.64% sodium hypochlorite - 10% ethanol)
- Sterile deionized water
Equipment
- Petri dishes (9 and 14 cm diameter)
- Filter paper (Whatman No.3, 8 and 12.5 cm diameter)
- Pipette tip or cork borer
- Sealing film (Phyto Technology Laboratories) or parafilm
- Ruler
- Razor blades
- Laminar flowhood
- Illuminated incubator at 21 ± 1 °C, light intensity > 90 μmols
- Fungal growth cabinet with white (one 18 W lamp) and black fluorescent (one 18 W lamp) lights on 12/12 h day/night cycle at 20-22 °C
- Incubator
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Covarelli, L., Gardiner, D., Beccari, G. and Nicholson, P. (2013). Fusarium Virulence Assay on Wheat and Barley Seedlings. Bio-protocol 3(7): e446. DOI: 10.21769/BioProtoc.446.
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分类
微生物学 > 微生物-宿主相互作用 > 体内实验模型 > 植物
植物科学 > 植物免疫 > 病害生物测定
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