在监视和受限小鼠中测定气道反应性

François Daubeuf; Laurent Reber; Nelly Frossard

doi:10.21769/BioProtoc.328

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Peer-reviewed

Measurement of Airway Responsiveness on Vigil and Unrestrained Mouse

在监视和受限小鼠中测定气道反应性

FD François Daubeuf

LR Laurent Reber

NF Nelly Frossard email

发布: 2013年02月20日第3卷第4期 DOI: 10.21769/BioProtoc.328 浏览次数: 9307

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参见作者原研究论文

The authors used this protocol in:

Cover of The Journal of Immunology, featuring study using the protocol.

Apr 2012

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Abstract

Airway hyperresponsiveness to methacholine is an important characteristic of asthma. This protocol describes how to measure airway response to methacholine in vigil and unrestraint mouse. The enhanced pause (PenH) is an index of the airway response to increasing doses of methacholine recorded by whole-body barometric plethysmography.

Keywords: Airway responsiveness (气道反应性)

Plethysmography (体积描记法)

PenH (金边)

Respiratory parameters (呼吸参数)

Mouse (小鼠)

Materials and Reagents

Sterile saline (0.9% NaCl)
Methacholine (Sigma-Aldrich, catalog number: A2251 )
Drierite (Sigma-Aldrich, catalog number: 238988 )
Nebulization (see Recipes)

Equipment

Whole body plethysmography system (EMKA Technologies), including standard setup (whole-body plethysmograph, differential pressure transducer, ventilation pump, amplifier, interface box, acquisition card and iox2 software with the respiratory flow analyzer module) and standard nebulisation equipment (LS230, SYSTAM, Villeneuve-sur-Lot)

Procedure

English

中文翻译

文章信息

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如何引用

Daubeuf, F., Reber, L. and Frossard, N. (2013). Measurement of Airway Responsiveness on Vigil and Unrestrained Mouse. Bio-protocol 3(4): e328. DOI: 10.21769/BioProtoc.328.