Organotypic Slice Culture of Embryonic Brain Sections
胚胎脑切片的培养
发布: 2013年02月05日第3卷第3期 DOI: 10.21769/BioProtoc.327 浏览次数: 17346
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参见作者原研究论文
The authors used this protocol in:
Jul 2012
Abstract
This technique will allow using brain slices to study several aspects of cortical development (i.e. neurogenesis), as well as neuronal differentiation (i.e. neuronal migration, axon and dendrite formation) in situ. This protocol is suitable for various embryonic stages (Calderon de Anda et al., 2010; Ge et al., 2010).
Keywords: Neuronal development (神经元发育)
Cortical development (皮质发育)
Neuronal migration (神经元的迁移)
Materials and Reagents
- Agarose Type VII (low melting) (Sigma-Aldrich, catalog number: A9045-100G )
- Neurobasal (Life Technologies, Gibco®, catalog number: 21103-049 )
- B27 (Life Technologies, InvitrogenTM, catalog number: 17504-04 )
- Glutamine (Life Technologies, Gibco®, catalog number: 25030 )
- Penicillin/streptomycin (Sigma-Aldrich, catalog number: P4458 )
- Horse serum (Life Technologies, InvitrogenTM, catalog number: 16050-122 )
- N2 (Life Technologies, InvitrogenTM, catalog number: 17502-048 )
- 1x PBS
- Krazyglue or similar glue
Equipment
- Vibratome (Leica, model: VT1000 S)
- Millicell, sterilized culture plate insert (EMD Millipore)
- Dissecting tools (Ring forceps, Serrated forceps, Fine scissors)
- Curved spatula
- 30 mm petri dish
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Anda, F. C. D. (2013). Organotypic Slice Culture of Embryonic Brain Sections. Bio-protocol 3(3): e327. DOI: 10.21769/BioProtoc.327.
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分类
发育生物学 > 细胞生长和命运决定 > 神经元
神经科学 > 发育 > 神经元
细胞生物学 > 组织分析 > 组织分离
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