Materials and Reagents

1. Purified protein as ligand
   
2. Synthetic peptide dissolved in DMSO as analyte
   
3. NaCl
4. NaOH
   
5. HEPES
   
6. Tween-20
   
7. 50 mM NaOH
   
8. Amine coupling kit {750 mg EDC (N-ethyl-N’-(3-dimethylaminopropyl)carbodiimide hydrochloride), 115 mg NHS (N-hydroxysuccinimide), 10.5 ml ethanolamine-HCl; GE Healthcare, catalog number: BR-10000-50 }
   
9. 10 mM Acetate buffer pH 4 to 5.5 (GE Healthcare, catalog number: BR-1003-49 to 52 )
   
10. DMSO (high grade, such as Sigma-Aldrich, catalog number: 276855 , or Sigma-Aldrich, catalog number: D-1435 )
    
11. MilliQ Water
12. Reagents for immobilization (see Recipes)
13. Reagents for binding assay (see Recipes)
14. Ligand (see Recipes)
15. Analyte (see Recipes)
    

Equipment

1. CM5 sensor chip (research grade; GE Healthcare, catalog number: BR-1003-99 )
2. Biacore X100 Evaluation Software (GE Healthcare)
   

Procedure