Materials and Reagents

1. Mito-miniSOG construct* driven by your chosen promoter, e.g., Pnmr-1-mito-miniSOG (interneuron) (http://www.wormbase.org provides general information for C. e. specific promoters. http://chinook.uoregon.edu/neuron.html provides a list of neuronal promoters)
   * Requests for miniSOG construct should be addressed to Roger Y. Tsien (UC San Diego).
   
2. (optional) non-green fluorescence marker to label your target cells or tissues, e.g., Pnmr-1-mCherry
   
3. Control construct that is free miniSOG driven by specific promoters, e.g., Pnmr-1-miniSOG
   
4. 100 mM CuCl₂
   
5. Filter paper
   
6. NGM (nematode growth media) plates (seeded and unseeded)
   
7. NGM (nematode growth  media) (see Recipes)
   

Equipment

1. Fluorescence stereomicroscope (dissecting microscope, e.g., Leica, model: M165 )
   
2. Upright epi-fluorescecne microscope (compound microscope, e.g., Zeiss Axio Imager 2)
   
3. Prizmatix LED (UHP-Mic-LED-460), and Digital Function Generator (PASCO Scientific, catalog number: PI-9587C )
   
4. Microinjection rig to generate transgenic worms
   

Procedure