After official publication of our protocol in bio-protocol (https://bio-protocol.org/e3046), we noted an error in the protocol.
The recipe for Phosphate-buffered saline (PBS) was in fact the one for 10x PBS. Accordingly, we need to make corrections as follows (in Recipes):
“1. Phosphate-buffered saline (PBS)” → “1. 10x Phosphate-buffered saline (10x PBS)”.
“Add ddH₂O to prepare 1 L PBS” → “Add ddH₂O to prepare 1 L 10x PBS. Dilute 10 times with ddH₂O to prepare 1x PBS”.

References

1. Ke, M. and Imai, T. (2018). Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2. Bio-protocol 8(20): e3046.