马铃薯嫁接实验

Paula  Suárez-López

doi:10.21769/BioProtoc.303

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Peer-reviewed

Grafting of Potato Plants

马铃薯嫁接实验

PS Paula Suárez-López email

发布: 2012年12月05日第2卷第23期 DOI: 10.21769/BioProtoc.303 浏览次数: 11733

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参见作者原研究论文

The authors used this protocol in:

Cover of The Plant Journal, featuring study using the protocol.

May 2012

实验方案合集

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Abstract

Grafting is a very useful technique for testing the transmission of long-distance signals in plants and is used in agriculture and gardening for different purposes. This protocol, based on a previously published one (Jackson et al., 1998), describes a grafting method for potato plants, which has successfully been used to test the transmission of tuber-inducing signals (Martin et al., 2009; González-Schain et al., 2012). We describe first the procedures for plant growth and then the grafting procedure. Although this method has been used for grafting plants that were initially grown in vitro, it should work as well with plants grown from tubers in soil. This protocol probably works well for other plant species, with small modifications.

Keywords: Grafting (嫁接)

Potato (马铃薯)

Solanum tuberosum (马铃薯)

Materials and Reagents

Murashige & Skoog medium including vitamins (Duchefa Biochemie, catalog number: M0222 )
Sucrose
MES (Sigma-Aldrich, catalog number: M8250 )
ddH₂O
KOH
Gelrite^TM (Duchefa Biochemie, catalog number: G1101.5000 )
Soil composed of blocking compost (Plantaflor^® Presstopfsubstrat) and sand in a 3:1 proportion
KNO₃
NH₄NO₃
K₂HPO₄
KH₂PO₄
Ca(NO₃)₂^.4H₂O
MgSO₄₂^.7H₂O
FeSO₄₂^.7H₂O
Kelamix
Fertilization solution: modified Hoagland’s solution (Johnson et al., 1957) diluted 1/60 (see Recipes)
2MS medium (see Recipes)

Equipment

Plant growth chamber
Laminar flow hood
Sterile forceps
Glass jars, glass tubes or plastic containers suitable for in vitro growth
Note: Equipments 1-4 is not required if plants are grown from tubers in soil.
Sterile scalpels
Pots
Stakes
Plant twist ties (e.g. Garden Odyssey, catalog number: T001 )
Beaker
Paper surgical tape (e.g. 3 M Micropore medical tape, catalog number: 1530-0 )
Transparent plastic bags
Adhesive tape