Plastic Embedding and Sectioning of Plant Tissues
植物组织树脂包埋与切片
发布: 2012年12月05日第2卷第23期 DOI: 10.21769/BioProtoc.296 浏览次数: 17501
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参见作者原研究论文
The authors used this protocol in:
Feb 2011
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Abstract
Plastic (resin) embedding provides exclusively improvements to cellular definition compared to paraffin embedding. The combination of strongly cross-linking paraformaldehyde with glutaraldehyde and post fixed with OsO4 is the fixative of choice for high-resolution light microscopy and electron microscopy. For this reason, this method is an ideal tool for visualizing plant cellular morphology and phenotype.
Materials and Reagents
- Ethanol
- Acetone
- Na2HPO4
- Gelatin capsules (Electron Microscopy Sciences, catalog number: 71012 )
- Glutaraldehyde (Fluka, catalog number: 49627 )
- Paraformaldehyde
- OsO4
- Catalyst
- Osmium (Fluka, catalog number: 75633 )
- Fixative solution (see Recipes)
- Post-fixative solution (see Recipes)
- NaPO4 buffer (see Recipes)
- Resin (LR white) (Fluka, catalog number: 62662 ) (see Recipes)
Equipment
- Shaker
- Incubator with temperature control
- Light or electron microscopy
- Vacuum
- Glass blade or jeweler saw (Electron Microscopy Sciences, catalog number: 71012 )
Procedure
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文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Liu, T. (2012). Plastic Embedding and Sectioning of Plant Tissues. Bio-protocol 2(23): e296. DOI: 10.21769/BioProtoc.296.
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分类
植物科学 > 植物细胞生物学 > 组织分析
细胞生物学 > 组织分析 > 组织染色
植物科学 > 植物细胞生物学 > 组织分析
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