植物组织树脂包埋与切片

Tie Liu

doi:10.21769/BioProtoc.296

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Peer-reviewed

Plastic Embedding and Sectioning of Plant Tissues

植物组织树脂包埋与切片

Tie Liu

发布: 2012年12月05日第2卷第23期 DOI: 10.21769/BioProtoc.296 浏览次数: 16840

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The authors used this protocol in:

Cover of Micron, featuring study using the protocol.

Feb 2011

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Abstract

Plastic (resin) embedding provides exclusively improvements to cellular definition compared to paraffin embedding. The combination of strongly cross-linking paraformaldehyde with glutaraldehyde and post fixed with OsO₄ is the fixative of choice for high-resolution light microscopy and electron microscopy. For this reason, this method is an ideal tool for visualizing plant cellular morphology and phenotype.

Materials and Reagents

Ethanol
Acetone
Na₂HPO₄
Gelatin capsules (Electron Microscopy Sciences, catalog number: 71012 )
Glutaraldehyde (Fluka, catalog number: 49627 )
Paraformaldehyde
OsO₄
Catalyst
Osmium (Fluka, catalog number: 75633 )
Fixative solution (see Recipes)
Post-fixative solution (see Recipes)
NaPO₄buffer (see Recipes)
Resin (LR white) (Fluka, catalog number: 62662 ) (see Recipes)

Equipment

Shaker
Incubator with temperature control
Light or electron microscopy
Vacuum
Glass blade or jeweler saw (Electron Microscopy Sciences, catalog number: 71012 )

Procedure

English

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Liu, T. (2012). Plastic Embedding and Sectioning of Plant Tissues. Bio-protocol 2(23): e296. DOI: 10.21769/BioProtoc.296.