Measurement of Hemoglobin
血红蛋白检测
发布: 2012年11月20日第2卷第22期 DOI: 10.21769/BioProtoc.291 浏览次数: 18915
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参见作者原研究论文
The authors used this protocol in:
Mar 2012
Abstract
This protocol allows to measure the levels of intratumoral hemoglobin from human or rodent fresh samples but also frozen tumors. The advantage of this method is to use very few microliters of biological material for hemoglobin and the protocol is carried out quickly.
Keywords: Cancer (癌症)
Angiogenesis (血管生成)
Resistance to treatment (治疗抵抗)
Materials and Reagents
- Fresh or frozen tumor tissues
- Extraction buffer (Life Technologies, catalog number: FNN0011 )
- Human hemoglobin (Sigma-Aldrich, catalog number: H7379 )
- Drabkin's reagent (Sigma-Aldrich, catalog number: D5941 )
- Brij 35 Solution 30% (Sigma-Aldrich, catalog number: B4184 )
- Antifoam Y-30 Emulsion (Sigma-Aldrich, catalog number: A5758 )
- BCA protein quantification kit (Interchim, catalog number: MP2920 )
- Extaction buffer
Equipment
- Homogenizer such as Precellys (Ozyme BER1011S, France) or ultraturax
- 96 wells plates (DUTSCHER SCIENTIFIC, catalog number: 047632 )
- Luminoskan (Thermo Fisher Scientific, catalog number: 5210470 )
- Centrifuges
Procedure
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Grépin, R. and Pagès, G. (2012). Measurement of Hemoglobin. Bio-protocol 2(22): e291. DOI: 10.21769/BioProtoc.291.
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分类
癌症生物学 > 通用技术 > 生物化学试验 > 蛋白质分析
生物化学 > 蛋白质 > 分离和纯化
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