Mast Cell Dependent Airway Hyperresponsiveness (AHR)
肥大细胞导致的气道高反应性小鼠模型(AHR)
发布: 2012年10月05日第2卷第19期 DOI: 10.21769/BioProtoc.267 浏览次数: 11342
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参见作者原研究论文
The authors used this protocol in:
Feb 2012
Abstract
Asthma is a complex phenotype that involves multiple mechanisms, including adaptive and innate immunity as well as physiological and mechanical changes in the airways. In the models of asthma induced by sensitization and aerosolized allergen exposure in the absence of adjuvant, mast cells facilitate the development of in ammation and airway hyper-responsiveness. This model is useful to analysis of function of mast cells in AHR.
Materials and Reagents
- Mice (C57BL/6)
Note: BALB/c is also appropriate to this experiment.
- Ovalbumin (OVA) (grade V) (Sigma Aldrich, catalog number: A5503 )
- Pentobarbital (Sigma-Aldrich, catalog number: P3761 )
- Acetylcholine chloride (Sigma-Aldrich, catalog number: A6625 )
- Phosphate buffered saline (PBS) (Life Technologies, Gibco®, catalog number: 20012 )
- Wright–Giemsa stain (MUTO PURE CHEMICALS CO.,LTD, catalog number: 15022 )
- Saline
Equipment
- Syringe (1 ml) (Terumo Medical Corporation, catalog number: SS-01T2525 )
- Needle (26G1/2)
- Ultrasonic nebulizer (NIHON KOHDEN CORPORATION, catalog number: TUR-3200 )
- Differential pressure transducer (NIHON KOHDEN CORPORATION, catalog number: TP-602T )
- Light microscope (OLYMPUS, model: CX-41 )
- Tracheal cannula (Natume, catalog number: SP31 )
- Cytospin (Shandon, model: Cytospin 3 )
- Centrifuges
Procedure
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Motomura, Y. and Kubo, M. (2012). Mast Cell Dependent Airway Hyperresponsiveness (AHR). Bio-protocol 2(19): e267. DOI: 10.21769/BioProtoc.267.
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分类
免疫学 > 动物模型 > 小鼠
细胞生物学 > 细胞染色 > 全细胞
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