Mast Cell Dependent Airway Hyperresponsiveness (AHR)

Materials and Reagents

1. Mice (C57BL/6)
   Note: BALB/c is also appropriate to this experiment.
   
2. Ovalbumin (OVA) (grade V) (Sigma Aldrich, catalog number: A5503 )
   
3. Pentobarbital (Sigma-Aldrich, catalog number: P3761 )
   
4. Acetylcholine chloride (Sigma-Aldrich, catalog number: A6625 )
   
5. Phosphate buffered saline (PBS) (Life Technologies, Gibco^®, catalog number: 20012 )
   
6. Wright–Giemsa stain (MUTO PURE CHEMICALS CO.,LTD, catalog number: 15022 )
   
7. Saline
   

Equipment

1. Syringe (1 ml) (Terumo Medical Corporation, catalog number: SS-01T2525 )
   
2. Needle (26G1/2)
   
3. Ultrasonic nebulizer (NIHON KOHDEN CORPORATION, catalog number: TUR-3200 )
   
4. Differential pressure transducer (NIHON KOHDEN CORPORATION, catalog number: TP-602T )
   
5. Light microscope (OLYMPUS, model: CX-41 )
   
6. Tracheal cannula (Natume, catalog number: SP31 )
   
7. Cytospin (Shandon, model: Cytospin 3 )
   
8. Centrifuges
   

Procedure