In vivo Matrigel Plug Angiogenesis Assay
基质胶内血管生成实验
发布: 2012年09月20日第2卷第18期 DOI: 10.21769/BioProtoc.261 浏览次数: 51671
评审: Ivan ZanoniAlexandros AlexandratosAnonymous reviewer(s)
下载 PDF 
Q&A
参见作者原研究论文
The authors used this protocol in:
Feb 2012
Abstract
The matrigel plug angiogenesis assay is a simple in vivo technique to detect the newly formed blood vessels in the transplanted gel plugs in nude mice. The matrigel matrix is derived from the engelbroth-holm-swarm (EHS) mouse sarcoma, and its composition is comparable to the basement membrane proteins. The matrigel can induce differentiation of a variety of cell types such as hepatocytes, mammary epithelial cells, and endothelial cells. In our case, tumor cells are mixed with the matrigel gel and are injected into the mice. The later immunohistochemistry (IHC) staining with the endothelial marker indicates the presence of the newly formed capillaries in the sectioned gel plugs.
Keywords: In vivo (在体内)
Matrigel plug (Matrigel plug)
Angiogenesis (血管生成)
Nude mice (裸鼠)
Endothelial cell (内皮细胞)
Materials and Reagents
- BALB/cAnN-nu (Nude) female mouse, 6 to 8 week-old
- Tumor cells
- Matrigel matrix (BD Biosciences, Falcon®, catalog number: 354234 )
- 10% formalin solution-neutral buffered (Sigma-Aldrich, catalog number: HT501128-4L )
- Anti-rat CD34 (Santa Cruz, catalog number: sc-18917 )
- Biotin goat Anti-Rat IgG (BD Biosciences, catalog number: 559286 )
- Streptavidin-peroxidase conjugate (Dako, catalog number: P0397 )
- Diaminobenzidine (DAB) (Dako, catalog number: K3467 )
- Paraffin
- Potassium chloride
- Potassium phosphate monobasic
- Dodium chloride (NaCl)
- Sodium phosphate dibasic anhydrous
- Hematoxylin and eosin (H&E)
- Trypsin
- Complete medium
- Plain medium
- Phosphate buffered saline (PBS) (see Recipes)
Software
- Aperio ScanScope System
- ImageScope v10 software (Aperio, Vista)
Equipment
- Aperio Scanscope CS-S microscopic slide scanning system
- Tumor cell culture set up
- Hemocytometer
- Centrifuges
- 24G syringe
- T175 flask
Procedure
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Lung, H. L. and Lung, M. L. (2012). In vivo Matrigel Plug Angiogenesis Assay. Bio-protocol 2(18): e261. DOI: 10.21769/BioProtoc.261.
Download Citation in RIS Format
分类
癌症生物学 > 血管生成 > 动物模型
您对这篇实验方法有问题吗？
在此处发布您的问题，我们将邀请本文作者来回答。同时，我们会将您的问题发布到Bio-protocol Exchange，以便寻求社区成员的帮助。
提问指南
+ 问题描述
写下详细的问题描述，包括所有有助于他人回答您问题的信息（例如实验过程、条件和相关图像等）。
发布问题
