Immunogold Localization of Molecular Constituents Associated with Basal Bodies, Flagella, and Extracellular Matrices in Male Gametes of Land Plants

Background

Motile gametes of land plants are strikingly diverse with numbers of flagella ranging from two to greater than 40,000 (Renzaglia and Garbary, 2001). Following a series of synchronized mitotic divisions within antheridia, nascent sperm cells (spermatids) undergo a sequence of developmental changes within the confines of a dynamic and growing cell wall. A complex locomotory apparatus is produced and flagella elongate around the cell as the organelles are repositioned and shaped. Synchronized development yields hundreds of cells in a single stage of maturation and in different planes of section within a single antheridium.

This profound cellular differentiation involves the development of unique MT arrays, the spline and flagella, that emanate from discrete microtubule organizing centers (MTOCs), the only centriole-containing centrosomes in land plants. Because of the exclusive occurrence of basal bodies, flagella and associated complexes in developing male gametes, studies of spermatogenesis have revealed important information on the structure, composition, and developmental changes in MT arrays as they relate to the cell cycle, MTOCs and cellular differentiation in plants (Joshi et al., 1992; Lui et al., 1993; Vaughn and Renzaglia, 1993; Hoffman et al., 1994; Hoffman and Vaughn, 1995; Vaughn and Harper, 1998; Klink and Wolniak, 2003; Vaughn and Renzaglia, 2006; Vaughn and Bowling, 2008; Vaughn, 2013). A dynamic and flexible extracellular matrix is necessary for spermatogenesis to take place (Garbary and Renzaglia, 2001; Lopez and Renzaglia, 2014); thus spermatogenesis in plants provides an opportunity to examine cell wall changes during development.

The purpose of this review is to describe the methodologies used in localizing proteins, carbohydrates and glycoproteins during the development of motile gametes in land plants. One of the most powerful tools in these studies involves antibodies that recognize epitopes to macromolecules using immunogold labeling techniques at the TEM level (Vaughn, 2013). Here we provide images and a brief discussion of results using immunogold labeling to examine the molecular constituents involved in sperm cell development in plants. Two procedures for these investigations are provided that use the same Materials and Reagents, and Equipment: Procedure A describes the protocol for microtubule-related proteins and procedure B for localizing cell wall constituents.

Procedure A: Immunogold labeling has led to important advances in understanding the role of the proteins centrin and tubulin in plants (Figures 1A-1E). Centrin is a ~20 kDa Ca-binding protein first discovered in motile green algae (Satisbury, 1995), where it is localized to the stellate pattern in the transition zone of the flagella and a dense band of fibers (distal fibers) that connect the nucleus to the basal bodies. In spermatogenous cells, centrin localizes to specific, seemingly diverse structures (Figures 1A-1D). The plates of the multilayered structure (MLS) of the locomotory apparatus, but not the microtubules (MTs), are strongly labeled with antibodies that recognize centrin (Vaughn and Renzaglia, 1993; Vaughn and Harper, 1998) (Figures 1A and 1B). The transition zone that occurs in the flagella of most plants with motile cells also strongly labels with antibodies to centrin, indicating homology with a similar zone in the basal body apparatus in green algae (Vaughn and Renzaglia, 1993; Hoffman et al., 1994; Vaughn and Harper, 1998; Klink and Wolniak, 2003; Vaughn and Renzaglia, 2006) (Figures 1C and 1D). In tracheophytes, an electron opaque pericentriolar type material, called the amorphous zone (AZ), runs along the top of the spline (MT band), connects the basal bodies of adjacent flagella and labels with centrin antibodies (Figure 1B) (Hoffman et al., 1994; Hoffman and Vaughn, 1995). The AZ thus serves as that same sort of basal body connector as found in the green algae.

These localizations are indicative of two possible functions for centrin, as an MTOC protein, and as a contractile protein. In the MLS, centrin appears to be involved in MT nucleation and organization of the spline MT array. In the AZ and transition zone, it is more likely that the centrin is involved in contractile functions. The AZ might also be involved in nucleation/organization of MTs as it lies at the base of the basal body and is close to the spline MT array.

Gamma tubulin was the last of the tubulin proteins to be discovered (Oakley et al., 1990) and occurs in a much lower quantity than alpha and beta tubulin. In mammalian cells, gamma tubulin is restricted to the ends of MTs, where it forms a template for the MTs to form (Joshi et al., 1992). In contrast, gamma tubulin in plants occurs along MTs and not just at their termini (Liu et al., 1993; Liu et al., 1994; Hoffman et al., 1994; Vaughn and Harper, 1998). These sites are in fact new nucleating sites as plant MTs form more of a ‘fir tree’ or highly branched pattern than that is noted in mammalian cells (Murata et al., 2005; Murata and Hasebe, 2007).

The blepharoplast occurs in the last two spermatogenous cell divisions in pteridophytes and serves as the spindle pole body in these divisions as well as the template for basal body production (Hepler, 1976; Hoffman and Vaughn, 1995). In an attempt to determine its ability to nucleate MTs, Vaughn and Bowling (2008) treated Ceratopteris antheridia with the potent microtubule-disrupter oryzalin (ChemService Inc., West Chester, PA) leading to the loss of all microtubules except those in stabilized MT arrays such as in flagella. In these oryzalin-treated cells, the blepharoplast was clearly recognizable but free of MTs and covered with pits that were the size and structure of the MT templates (tubulin ring complexes) recognized in mammalian cells.


Figure 1. Immunogold labeling of basal bodies and flagella of land plants. A. Centrin localization in the lamella strip (ls) that subtends the band of microtubules (mt) and basal bodies (b) of Phaeoceros carolinianus, a hornwort. B. In the seed plant, Ginkgo biloba, centrin epitopes are localized in the lamellar strip (ls), amorphous zone (az) where the basal bodies insert and stellate pattern (sp) of the transition zone. C. Longitudinal section of the long stellate pattern in Ceratopteris basal bodies that label with anti-centrin. A faint outline of the stellate pattern is visible at the arrow. D. Cross section at the basal body of Ceratopteris showing centrin localizations in the stellate pattern and amorphous zone around the basal body. E. In Ceratopteris spermatogenous cells, gamma tubulin (arrows) localizes around the periphery of the blepharoplast following oryzalin treatment. Bars = 0.1 µm.

Gamma tubulin antibodies label the periphery of the blepharoplast in oryzalin treated cells (Figure 1E). When the oryzalin is washed from the antheridia, MTs are quickly reformed along this pitted surface, further indicating the ability of the blepharoplast to serve as an MTOC.

In mammalian cells, the centrioles are surrounded by an electron opaque material where spindle MTs emanate. To identify the components of this pericentriolar material, monoclonal antibodies (MAbs) were raised to mitotic cells and MAbs that recognize the centriolar material could be used not only for mammalian cells but also for other materials, including spermatogenous cells. For example, MPM-2 recognizes a phosphorylated-protein epitope (Davis et al., 1983; Vandre et al., 1984) in spermatogenous cells. In cells without blepharoplasts, this MAb recognizes the surface of the nuclear envelope immediately before mitosis (Hoffman et al., 1994; Klink and Wolniak, 2003). These are the sites where MTs appear to be produced prior to mitosis in all plant cells. In cells with a blepharoplast, these antibodies strongly label the interior of this structure, not the edges (Hoffman et al., 1994; Vaughn and Bowling, 2008). Interestingly, as the blepharoplast begins to reorganize, the reactivity of the antibody is lost and centrin labeling increases in the pericentriolar material. Thus, as different MT arrays are formed, changes occur in proteins of the MTOC.

Procedure B: Immunogold localizations of the sequential matrices that are made during spermatogenesis has revealed differential labeling of carbohydrate-specific MAbs during development and across phylogeny. Callose is a prominent wall constituent in spermatogenesis of ferns, especially in the thickened wall of rounded spermatids in the early stages of ontogeny. In this stage, the locomotory apparatus originates and consists of a multilayered structure (MLS) and basal bodies (Figure 2A). Pectin is absent in this thickened wall in ferns (Lopez and Renzaglia, 2017). In contrast, mosses have a comparable wall that is deposited as spermatids become round, but it is devoid of callose and contains scattered aggregates of esterified pectin as localized with the JIM7 MAb (Figure 2B). By far the most abundant polysaccharide in this thickened wall layer in mosses is hemicellulose that localizes with both LM15 and LM25 MAbs (Figures 2C and 2D) (Lopez-Swalls, 2016).

In addition to carbohydrates, the walls involved in plant spermatogenesis contain abundant but diverse arabinogalactan proteins (AGPs) (Figures 2E-2G). AGPs recognized by the LM2 MAb replace the hemicelluloses around moss spermatids (Figure 2E). As the spermatid matures and begins to develop flagella and assume a coiled configuration, a flexible extraprotoplasmic matrix forms between the plasmalemma and thick callosic wall in ferns and between the plasmalemma and hemicellulosic-pectinaceous wall of mosses (Figures 2F and 2G). The matrix does not label with monoclonal antibodies raised against standard cell wall polysaccharide epitopes such as pectins, cellulose, and hemicelluloses. Rather, MAbs that recognize sugar residues of AGPs abundantly label the matrix as well as the plasmalemma of elongating flagella in fern and moss spermatids (Figures 1F and 1G) (Lopez and Renzaglia, 2014). These results coupled with light and fluorescence microscopy and inhibitor experiments with Yariv (a reagent that binds and precipitates AGPs) suggest that AGPs are involved in growth and positioning of flagella. The implication of AGPs as calcium modulators through binding and release of Ca²⁺ (Lamport and Várnai, 2013) is a potential mechanism for the regulation of cellular development in plants, and spermatogenesis is an ideal system in which to further pursue this hypothesis.


Figure 2. Immunogold labeling of spermatogenous cell walls in land plants. A. Callose localization in the unevenly-thickened wall layer that surrounds spermatids of Ceratopteris richardii during the formation of the locomotory apparatus which includes an anterior mitochondrion (am), a multilayered structure (mls) and basal bodies (b). B. A thickened wall layer comparable to that in Ceratopteris spermatids, is deposited by young spermatids in the moss, Physcomitrella patens. This wall labels intensely with the JIM7 MAb that binds to esterified pectin epitopes. C-D. Immunogold labeling of hemicelluloses in the thickened wall layer of young spermatids in the moss Aulacomnium palustre. C. This wall layer contains abundant xyloglucan epitopes recognized by the LM15 MAb. D. Similarly, galactoxyloglucan epitopes (LM25 MAb) are a rich component of these thickened walls. (Note: Spermatids in C. were post-fixed in osmium tetroxide (OsO₄) that reveals the loose fibrillar consistency of the wall compared to the spermatids in D. that were not post-fixed in OsO₄.) E-G. Immunogold labeling of arabinogalactan proteins (AGPs) in spermatid walls. E. AGP epitopes recognized by the LM2 MAb replace the hemicelluloses in the wall around spermatids in P. patens. F. JIM13, a monoclonal antibody to the epitope structure (β)-D-GlcpA1-(1,3)-α-D-GalpA-(1,2)-L-Rha of AGPs, is expressed in the extracellular matrix (*) around flagella during development in C. richardii. The microtubule band (mt), basal body (b) and a hub extension (h) are visible inside the developing spermatid. G. Cross sections of Ceratopteris flagella labelled with JIM8, a monoclonal antibody that identifies an unknown AGP epitope, showing specific localization on the plasmalemma. Bars = 0.5 µm for A-E; 0.1 µm for F-G.